可可体细胞胚胎发生及植株再生体系的构建

以可可（TheobromaCac0L.）的子叶为外植体，通过体胚发生途径，诱导再生可可植株。各培养阶段的优化培养基和培养条件为∶（1）愈伤组织诱导培养基（PCG）∶改良DKW+2，4-D3.0mgL+KT1.0mg几+TDZ 0.01mgL，在28+2）℃（以下培养温度均同）温度条件下，暗培养20d，诱导率为96.67%;（2）愈伤组织增殖培养基（SCG）∶改良DKW＋2，4-D 3.0mgA+KT1.0mg九，暗培养20d;（3）胚状体诱导培养基（ED）∶改良DKW+Sucrose30gL，暗培养60~150d，胚状体诱导产生并发育成熟，胚状体的诱导率为333%;（4）成熟胚诱导成苗∶①PEC培养基为∶改良DKW+Ghrose20gl+Sucrose10gL，光照为16h/d，培养60d;②采用RD培养基∶改良DKW+BA1.5mgl+AA0.5mgl，光照为16h/，培养3090d后，可得到完整的植株，再生植株的诱导率为 42%。

Study on Somatic Embryogenesis and Plant Regeneration of Theobroma Cacao L.

Plants were regenerated through somatic embryogenesis using cotyledon as explants. The optimized medium and condition for each stage were tested as folows,(1)Primary calli medium (PCG) was the improved DKW with 3.0mg/Lof 2,4-D,1.0mg/L of KT and0.01mg/L.of TDZ,explants were cultured on PCG medium in the dark at (28±2)℃ for 20 days.(2)Cal propagation medium (SCG)was the improved DKW with 3.0mg/L.of 2, 4-D,1.0mg/L. of KT, the alli were cultured on SCG medium for 30 days in the dark. (3)The call were subcultured on ED medium with 30g/L of sucrose,1g/L of glucose in the dark for 60 to 150 days,and embryos at different developmental stages(globular,beart torpedo and mature) were present on individual explants at the same time.(4)The mature embryos were transfered to fresh PEC medium every 30 days unil shoots with one or two leaves developed.The contents ofPEC medium were the improved DKW with 20g.Lof glucose,10g/L of sucrse. Shoot -producing embryos with 2 leaves were transfered onto RD medium that was the improved DKW suplemented with 1.5 mg/Lof IBA and 0.5 mg/LofIAA.The culures were maitained underlight for 16 b/d. Afer 30 to 90 days,regenerated plants were obtained