广东松种子若干生理指标的测定

[目的]测定广东松种子若干生理指标,以期为国家保护植物广东松的保育提供基础资料。[方法]种子含水量采用加热烘干称重法测定;可溶性糖含量采用蒽酮试剂法测定;氨基酸含量采用茚三酮试剂和抗坏血酸比色法测定;蛋白质含量采用考马斯亮蓝法测定;发芽率和发芽势采用萌发法测定。发芽率的快速测定分别采用氯化三苯基四氮唑法（TTC法）和红墨水染色法;萌发指数同发芽率测定方法;种子活力采用电导仪法测定。[结果]广东松种子相对含水量10.33%,可溶性糖含量6.35%,氨基酸含量4387.25μg/g,蛋白质含量77.67μg/ml,发芽率21.11%;发芽期间,26～35d中每间隔2d发芽势为1.11～7.78;萌发指数2.13;快速测定种子,采用TTC法、红墨水法测定活种子的发芽率,分别为94.44%、93.33%;相对电导率13.09%。[结论]种子相对含水量低,有利于种子保持活力和延长寿命。氨基酸和蛋白质含量较高,营养价值好。发芽率和萌发指数较低,体现出种子萌发明显受抑制,但新鲜种子大多具活力。电导率低,说明种子对逆境的抗性强。

Determination of Some Physiological Indexes of Pinus kwangtungensis Seed

[Objective]In order to provide some basic data for the national key protected plant species of Pinus kwangtungensis,some physiological indices of the seed were determined. [Method]The water content,the soluble sugar content,the amino acid content,the protein content were determined by using the method of drying and weight,anthrone reagent,ninhydrin reagent and antiscorbic acid,and coomassie brilliant blue,respectively. The germination rate and germination trend of the seed were determined by using the germi...