A bymovirus PCR primer and partial nucleotide sequence provides further evidence for the recognition of rice necrosis mosaic virus as a bymovirus |
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| Authors: | J.L. Badge S. Kashiwazaki S. Lock G.D. Foster |
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| Affiliation: | (1) Department of Botany, University of Leicester, Leicester, LE1 7RH, UK;(2) National Agriculture Research Center, Tsukuba, 305, Japan;(3) School of Biological Sciences, University of Bristol, Woodland Rd, Bristol, BS8 1UG, UK |
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| Abstract: | Rice necrosis mosaic virus (RNMV) has physico-chemical and biological properties typical of the Bymovirus genus. However, to date no sequence data has been reported for RNMV. In this paper we report the use of a PCR primer, designed from an area of high homology between bymoviruses and related macluraviruses within the NIb gene, which generated a 1.4kb fragment from the 3' terminal region of RNA 1 of RNMV. The sequence data generated from this region confirmed the classification of RNMV as a member of the Bymovirus genus. |
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