| 家蚕中肠酸性磷酸酶细胞化学的研究 |
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| 引用本文： | 陈玉银,黎军英,吴玉澄.家蚕中肠酸性磷酸酶细胞化学的研究[J].蚕业科学,1999,25(3):154-157. |
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| 作者姓名： | 陈玉银 黎军英 吴玉澄 |
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| 作者单位： | 浙江大学蚕学系,杭州,310029 |
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| 摘 要： | 应用超微结构酶细胞化学技术研究了家蚕幼虫中肠ＡＣＰａｓｅ 活性的亚细胞分布，结果表明ＡＣＰａｓｅ 主要分布在圆筒形细胞的细胞核内，核周围的内质网及溶酶体中，杯形细胞的细胞核、细胞底部的内褶膜上也有少量的酶活性存在。不同数量性状的蚕品种间圆筒形细胞细胞核内的ＡＣＰａｓｅ 活性分布有显著差异。
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| 关 键 词： | 家蚕中肠 酸性磷酸酶 超微结构 细胞化学定位 |
| 修稿时间： | 1998－10－12 |
Cytochemical Studies on the Localization of Acid Phosphatase Activity in Midgut Epithelium of Silkworm, Bombyx mori |
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| Chen Yuyin,Li Junying,Wu Yucheng.Cytochemical Studies on the Localization of Acid Phosphatase Activity in Midgut Epithelium of Silkworm, Bombyx mori[J].Acta Sericologica Sinica,1999,25(3):154-157. |
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| Authors: | Chen Yuyin Li Junying Wu Yucheng |
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| Abstract: | Acid phosphatase(ACPase) in midgut epithelium of silkworm larvae was studied ultrastructurally.The results showed that the heavy lead phosphate deposits indicating a high activity of ACPase were obviously localized at chromatin of nuclei in columnar cells,and reaction products also showed that lysosomes and endoplasmic reticulum were ACPase activity positive.Nuclei of goblet cells,along the nuclear membrane and basal plasma membrane were also slightly ACPase positive.Silkworm variety with higher quantitative character showed stronger ACPase activity in the nucleus of columnar cells.The characteristics of enzyme activity distribution in silkworm larvae was considered to be related to the special function of columnar cells in silkworm larvae. |
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| Keywords: | Bombyx mori midgut ACPase Ultrastructure Cytochemical localization |
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