屠宰猪肝脏组织中戊型肝炎病毒的检测

摘要：戊型肝炎是由戊型肝炎病毒（hepatitis E virus ，HEV）引起的一种人兽互传病毒性肝炎，主要经消化道传播，猪等多种与人类密切接触的动物均可感染。为了解屠宰商品猪肝脏中HEV的感染情况，从北京、河北和河南的屠宰场共采集了581头屠宰猪的肝脏，猪源分别来自黑龙江、吉林、辽宁、河北、北京等地的集约化猪场，河南的个体散养户和集约化猪场饲养的商品猪。每头猪取样两份，一份固定，做石蜡切片，用抗-人HEV抗体做肝脏中HEV免疫组织化学染色，另一份冷冻保存，提取病毒核酸，进行RT-PCR。结果发现，六个地区的屠宰猪肝脏HEV免疫组织化学阳性率除了河南散户为33.3%外，其它都在90%以上，最高达到100%。应用针对HEV ORF2区设计的通用引物，从部分样品中检测到了HEV核酸的存在。表明戊型肝炎在以上地区的商品屠宰猪中普遍存在，对猪肉品安全构成了威胁，应予以关注。

Detection of the hepatitis E Virus in liver of Slaughtered swine

Abstract: Hepatitis E is an enterically transmitted zoonosis caused by hepatitis E virus that infectious to many animals includes swine. To understand the current status of HEV infection in swine liver, 581 swine liver samples collected from Heilongjiang, Jilin, Liaoning, Hebei, Beijing and Henan province was examined by Immunohistochemistry stain、 H.E and RT-PCR. The result showed that HEV Immunohistochemistry stain positive rate was beyond 90%, except Henan(33.3%), the highest positive rate was 100%. With a pair of primer design from HEV ORF2 gene, Nucleic Acid of HEV can be detected in liver cells with PCR. It suggests that hepatitis E was widely prevalent in Heilongjiang, Jilin, Liaoning, Hebei, Beijing and Henan province .