| Cry2Ab杀虫蛋白对小地老虎幼虫致死效果及体内酶活性的影响 |
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| 引用本文： | 陆琼,张永军,于洪春,曹广春,陆宴辉,郭予元.Cry2Ab杀虫蛋白对小地老虎幼虫致死效果及体内酶活性的影响[J].植物保护学报,2009,36(1):16-20. |
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| 作者姓名： | 陆琼 张永军 于洪春 曹广春 陆宴辉 郭予元 |
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| 作者单位： | 陆琼,Lu Qiong(中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100193;东北农业大学农学院,哈尔滨,150030);张永军,曹广春,陆宴辉,郭予元,Zhang Yongjun,Cao Guangchun,Lu Yanhui,Guo Yuyuan(中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100193);于洪春,Yu Hongchun(东北农业大学农学院,哈尔滨,150030)
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| 基金项目： | 国家重点基础研究发展规划(973计划)，国家高技术研究发展计划(863计划) |
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| 摘 要： | 为治理小地老虎的危害,研究了Bt杀虫蛋白对小地老虎幼虫的控制作用.室内生物测定结果表明,Cry2Ab杀虫蛋白对小地老虎初孵幼虫的致死效果高于Cry1Ac杀虫蛋白.取食含有Cry2Ab杀虫蛋白人工饲料的小地老虎幼虫体内的中肠总蛋白酶和类胰凝乳蛋白酶活性高于对照,当人工饲料中Cry2Ab杀虫蛋白含量达到12μg/g时,小地老虎幼虫中肠氨肽酶活性显著升高;而取食含有Cry1Ac杀虫蛋白人工饲料的小地老虎幼虫中肠蛋白酶活性无显著变化.此外,小地老虎幼虫取食含有Cry2Ab杀虫蛋白的人工饲料后,体内谷胱甘肽-S-转移酶活性显著升高,而取食合有Cry1Ac杀虫蛋白的人工饲料后其变化不明显.
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| 关 键 词： | 小地老虎 Cry2Ab杀虫蛋白 中肠蛋白酶 |
| 收稿时间： | 2008/8/30 0:00:00 |
Insecticidal activity of Cry2Ab proteins to Agrotis ypsilon (Rottemberg) and induced protease activities changes in the larvae |
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| Lu Qiong,Zhang Yongjun,Yu Hongchun,Cao Guangchun,Lu Yanhui and Guo Yuyuan.Insecticidal activity of Cry2Ab proteins to Agrotis ypsilon (Rottemberg) and induced protease activities changes in the larvae[J].Acta Phytophylacica Sinica,2009,36(1):16-20. |
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| Authors: | Lu Qiong Zhang Yongjun Yu Hongchun Cao Guangchun Lu Yanhui and Guo Yuyuan |
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| Institution: | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China |
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| Abstract: | |
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| Keywords: | Agrotis ypsilon Cry2Ab insecticidal proteins midgut protease |
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