| 中华鳖气单胞菌菌细胞苗和菌化学成份疫苗研制 |
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| 引用本文： | 叶巧真. 中华鳖气单胞菌菌细胞苗和菌化学成份疫苗研制[J]. 水产学报, 2000, 24(2): 167-170 |
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| 作者姓名： | 叶巧真 |
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| 作者单位： | 1. 中山大学生命科学学院,广州,510275
2. 广东省东莞市水产研究所,511700 |
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| 基金项目： | 广东省东莞市1996年农业科研和推广项目! (甲鱼快速高产养殖与鳖病防治技术研究 ) ,广东农委 (1996) 4号 |
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| 摘 要： | 利用分离自中华鳖的嗜水气单胞菌Ａｈ９６１００４、Ａｈ９６０９１６和Ａｈ９７０５１６与温和气单胞菌Ａｓ９７０５１０菌体及其上清制备灭活苗细胞苗和化学成份疫苗。肌肉注射中华鳖进行免疫，加强免疫１５ｄ后，用菌株Ａｈ９６１００４腹腔注射进行攻击，观察２０ｄ。结果表明，温和气单胞菌Ａｓ９７０５１０株的菌苗及其胞外分泌物（ＥＣＰ）苗组和嗜水气单胞菌Ａｈ９６１００４株的外膜成份组对嗜水气单胞菌的攻击的保护率最高
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| 关 键 词： | 中华鳖 嗜水气单胞菌 菌细胞苗 化学成分疫苗 |
| 收稿时间： | 2014-04-17 |
| 修稿时间： | 2014-04-17 |
Preparation of inactivated whole -cell and cell chemical components of Aeromonas from Trinyx sinensis |
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| Ye Qiaozhen. Preparation of inactivated whole -cell and cell chemical components of Aeromonas from Trinyx sinensis[J]. Journal of Fisheries of China, 2000, 24(2): 167-170 |
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| Authors: | Ye Qiaozhen |
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| Affiliation: | School of Life Sciences,Zhongshan University, Guangzhou 510275 |
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| Abstract: | Inactivated whole-cell and cell chemical components were prepared with Aeromonas hydrophila Ah961004,Ah960916,Ah970516 and A.sobria As970510,Soft shell turtles,Trinyx sinensis,were immunized via intramuscular route,after 15d booster doses,challenged with strain Ah961004 intraperitoneally and observed for 20d,The results showed that the group of A.sobria As970510 inactivated whole-cell with its extracellular products and the group of A.hydrophila Ah961004 outer membrane components had highly protection against strain Ah961004, with the protection rate 87.5% and 75% respectively,the other groups were lower than 50%. |
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| Keywords: | Trinyx sinensis Aeromonas hydrophila Aeromonas sobria vaccine |
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