捕后虾夷扇贝闭壳肌免疫因子与活品品质评价初探

为探索采捕后活品贝类的品质变化规律,以虾夷扇贝Patinopecten yessoensis为对象,研究了其闭壳肌中酸性磷酸酶(ACP)、超氧化物歧化酶(SOD)、溶菌酶(LSZ)、过氧化氢酶(CAT)等4个非特异性免疫因子的变化规律,试验基于活品虾夷扇贝的商品流通链分别进行湿藏和干藏处理,捕后贮藏分为离水至甲板、运输船中转、港口卸货、基地缓冲池和净化等5个关键环节,共设10个采样点。结果表明:虾夷扇贝闭壳肌中表现出ACP及SOD活力,CAT活力很低,LSZ则未检出活力;在捕后106 h内,ACP活力呈现出先下降再升高的趋势,而SOD活力则呈先升高再下降再升高的趋势,二者与虾夷扇贝所受胁迫强度均有相关性。研究表明,闭壳肌中非特异性免疫因子ACP及SOD可以作为活品扇贝的胁迫应答因子及品质评价指标进行深入研究。

Immunologic factors in adductors and quality evaluation in live and air exposure and wet stored Yesso scollap Patinopecten yessoensis

To explore the quality change of post-harvested commercial bivalves, the metobolic profiles of Yesso scallop Patinopecten yessoensis were studied under stress during live storage. The regualr pattern of four nonspecific immunity factors,including acid phosphatase(ACP), superoxide dismutase (SOD), catalase(CAT) and lysozyme (LSZ) were monitored following the change of time. Wet storage and air exposure storage, two current commercial live storage ways, were carried out at five key links including boarding, transporting, landing, transition holding and purification. The results showed that,during the 106h of post-harvest, ACP activities were first declined and then increased and that SOD activities were first increased and then deceased, indicating that ACP and SOD have good connection with stress intensity of Yesso scallop. CAT activity was detected at a negligible level and LSZ activ-ity was not detected. In a word,nonspecific immunity factors, ACP and SOD activities in the adductor of scallop are needed for further investigation as stress-response indicators and for quality evaluation index of live product.