| 鲤鱼精蛋白的提取与抗菌稳定性研究 |
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| 引用本文： | 黄占旺,上官新晨,沈勇根,吴少福,徐明生,蒋艳. 鲤鱼精蛋白的提取与抗菌稳定性研究[J]. 农业工程学报, 2005, 21(2): 165-168 |
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| 作者姓名： | 黄占旺 上官新晨 沈勇根 吴少福 徐明生 蒋艳 |
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| 作者单位： | 江西农业大学食品科学系,南昌,330045;江西农业大学食品科学系,南昌,330045;江西农业大学食品科学系,南昌,330045;江西农业大学食品科学系,南昌,330045;江西农业大学食品科学系,南昌,330045;江西农业大学食品科学系,南昌,330045 |
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| 基金项目： | 江西省科技厅资助项目(赣科发计字［2003］188号) |
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| 摘 要： | 该文研究了采用超声波技术提取鲤鱼抗菌精蛋白的工艺条件和抗菌稳定性。实验结果表明：采用超声波技术提取鱼精蛋白硫酸用量少，提取时间短。用1.0A强度的超声波和5%的硫酸溶液提取鱼精蛋白，提取时间20 min，能得到较高活性的鲤鱼抗菌精蛋白。并进一步研究了pH值、温度、金属离子和蛋白酶对鲤鱼精蛋白抗菌活性的影响。
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| 关 键 词： | 鲤鱼精巢 鱼精蛋白 超声波 提取 抗菌稳定性 |
| 文章编号： | 1002-6819(2005)02-0165-04 |
| 收稿时间： | 2004-04-06 |
| 修稿时间： | 2004-04-06 |
Extraction of protamine from carp spermary and its antibacterial stability |
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| Huang Zhanwang,Shangguan Xinchen,Shen Yonggen,Wu Shaofu,Xu Mingsheng and Jiang Yan. Extraction of protamine from carp spermary and its antibacterial stability[J]. Transactions of the Chinese Society of Agricultural Engineering, 2005, 21(2): 165-168 |
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| Authors: | Huang Zhanwang Shangguan Xinchen Shen Yonggen Wu Shaofu Xu Mingsheng Jiang Yan |
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| Abstract: | The extraction of antibacterial protamine from carp spermary by ultrasonic technology was studied. The results show that H2SO4 concentration is low, and extracting time is also short by using ultrasonic technology. Protamine of higher antibacterial activity could be extracted with 1.0 A ultrasound, 5% H2SO4 for 20 min. And the effects of pH values, temperature, metal ion, protease on protamine antibacterial activity were also investigated. |
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| Keywords: | carp spermary protamine ultrasound extraction antibacterial stability |
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