| 铁皮石斛的组织培养与快速繁殖研究进展 |
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| 引用本文： | 宋顺 许奕 李敬阳 刘以道 覃和业 马蔚红. 铁皮石斛的组织培养与快速繁殖研究进展[J]. 中国农学通报, 2013, 29(33): 286-290. DOI: 10.11924/j.issn.1000-6850.2013-1799 |
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| 作者姓名： | 宋顺 许奕 李敬阳 刘以道 覃和业 马蔚红 |
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| 作者单位： | 1. 中国热带农业科学院海口实验站2. |
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| 基金项目： | 中国热带农业科学院科技开发启动资金 |
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| 摘 要： | 在介绍铁皮石斛的自然生长条件和繁殖状况的基础上，讨论了基本培养基、激素、天然添加物、碳源以及光照和温度等因素对铁皮石斛种子萌发、原球茎的增殖、原球茎的分化、生根与壮苗、移栽中的影响，阐述了近年来关于铁皮石斛组织培养的研究概况，并初步分析存在的问题及发展方向。
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| 关 键 词： | PCR扩增 PCR扩增 |
| 收稿时间： | 2013-07-01 |
| 修稿时间： | 2013-08-21 |
The research progress on the Tissue Culture and rapid multiplication of Dendrobium officinale |
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| Song Shun,Xu Yi,Li Jingyang,Liu Yidao,Qin Heye,Ma Weihong. The research progress on the Tissue Culture and rapid multiplication of Dendrobium officinale[J]. Chinese Agricultural Science Bulletin, 2013, 29(33): 286-290. DOI: 10.11924/j.issn.1000-6850.2013-1799 |
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| Authors: | Song Shun Xu Yi Li Jingyang Liu Yidao Qin Heye Ma Weihong |
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| Affiliation: | 1,2,3 (1Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou 570102; 2Hainan Key Laboratory of Banana Genetic Improvement, Haikou 570102; 3Seedlings Cultivation Center, Chinese Academy of Tropical Agricultural Sciences, Danzhou Hainan 571737) |
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| Abstract: | On the basis of introducting the Dendrobium officinale natural growth conditions and reproductive status,we discussed the impact of basal medium, hormones,natural additives,carbon source,light and temperature on seed germination,protocorm proliferation anddifferentitation,root growth,transplanting.We expounded the survey of the tissue culture in Dendrobium officinale and analysised preliminarily the problems and development direction. |
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| Keywords: | rapid multiplication |
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