甘肃肉牛CAST基因的多态性及其与肉质性状的相关性研究

为研究甘肃肉牛CAST基因的多态性及与肉质性状的相关性。利用PCR-RFLP对甘肃肉牛CAST基因多态性检测并利用最小二乘法拟合线性模型探索不同基因型与肉质性状间的关系。结果表明：在CAST基因3个SNP位点中发现T750C、A557G、G556T、351处DelGTT的T/C；相关分析表明：Hga I位点的各基因型与肉质性状均差异不显著；Hpy188III位点的剪切力值，AT基因型显著高于AA基因型；Acl I位点在剪切力值上Hh基因型显著高于HH基因型，HH基因型在失水率上显著高于hh基因型。鉴定了CAST基因是影响肉质性状的候选基因。

Analysis on Polymorphism of CAST Gene in Gansu Local Cattle and Its Relationships with Meat Quality Trait

Analysis on polymorphism of CAST gene in Gansu Local Cattle and its relationship with meat quality trait. Polymorphisms in CAST gene were identified with PCR-RFLP analysis in Gansu local cattle. The association between genotype markers and meat traits were estimated by least squares using a linear model that included effects of locus and genotype appropriate interactions. The results showed that Polymorphisms of the CAST gene were evidenced three SNP locus T750C, A557G ,G556T, and 351 InDelGTT T/C mutation. Analysis of CAST gene in the Hga I locus there was little evidence of genotypic differences in meat quality traits. Analysis of CAST gene in the Hpy188 III locus these cattles with the AT genotype had a greater shear force than that of AA. Analysis of CAST gene in the Acl I locus these cattles with the Hh genotype had a greater shear force than that of HH. Cattle with the HH genotype had a greater pressing loss percentage than that of hh. There was little evidence of genotypic differences in meat quality traits. CAST gene were established the theory of a candidate molecular marker for meat quality trait.