黑龙江大豆栽培品种和新品系对疫霉病抗性评价及抗病基因分析

本研究旨在筛选黑龙江地区的大豆疫霉病抗病品种和品系,并分析主要栽培品种所含有的抗病基因,为病害的防治和抗病品种的合理布局提供参考。在大豆苗期用下胚轴伤口接种方法对126个栽培大豆品种和135份大豆品系进行接种,鉴定其对黑龙江大豆主要疫霉病菌株（8个）的抗性。有72个品种抗4个以上菌株,占鉴定品种的57.1%,其中有2个品种抗菌株数最多（7个）,占抗性品种总数的2.8%。84份品系抗4个以上菌株,占鉴定品系的62.2%,其中有4个品系抗菌株数最多（7个）,占抗性品系总数的4.7%。对品种的基因分析表明,有30个大豆品种含有抗病基因,其中有10个品种分别含有Rps1k、Rps3a、Rps1c 3个主要基因。明确了黑龙江地区的126个大豆栽培品种和135份大豆品系对大豆疫霉主要菌株的抗病性,得出有30个大豆品种含有抗病基因,其中有10个品种分别含有Rps1k、Rps3a、Rps1c 3个主要基因。

Resistance Evaluation and Resistance Genes analysis of soybean cultivars and new lines in Heilongjiang province to Phytophthora root rot

Phytophthora root rot caused by Phytophthora sojae is one of main diseases in Heilongjiang soybean production. This disease have happened in the main soybean production areas of our country and led to a large quantity of outputs lose in some areas. Planting resistant cultivar is the most effective methods. So this research was focused on screening Phytophthora root rot resistant cultivars and resistance lines in Heilongjiang areas, and offered reference for controlling local disease and rational layout of disease-resistant cultivars. We used the hypocotyl vulnus inoculum to vaccinate 126 soybean cultivars and 135 portions of soybean lines, in order to identify eight main Phytophthora sojae strains. Our results showed that 72 portions of cultivars are resistant to more than 4 strains, occupy 57.1% of identified cultivars, and 84 portions of lines are resistant to more than 4 strains, occupy 62.2% of identified lines. Genes analysis of cultivars indicated that 30 soybean cultivars contained disease-resistant genes, 3 major genes, such as Rps1k, Rps3a and Rps1c, are included in 10 cultivars.