外源性孕酮对牛卵母细胞体外核成熟的影响

了研究外源性孕酮对牛卵母细胞体外核成熟的作用，通过向成熟培养液中添加10 μg/mL FSH +10 μg/mL LH（对照组）和0 ng/mL、10 ng/mL、100 ng/mL、1000 ng/mL的外源性孕酮，对牛卵丘卵母细胞复合体进行体外成熟培养，在培养8 h后用醋酸地衣红染色观察各自的生发泡破裂率，然后在成熟22 h统计第一极体率，体外受精后7~8天统计囊胚率。结果表明，对照组卵母细胞的生发泡破裂率(75.0%)和极体率(79.6%)均明显高于添加不同浓度孕酮的各处理组(P<0.05)，而囊胚率各组之间均无显著差异(P>0.05)。因此，外源性孕酮不能促进牛卵母细胞的核成熟，单独添加外源性孕酮不能起到与内源性孕酮相同的作用。

The Effect of Exogenous Progesterone on in vitro Nuclear Maturation of Bovine Oocytes

In this experiment,it was aimed to investigate the effect of exogenous progesterone on in vitro nuclear maturation of bovine oocytes through adding with 10 μg/mL FSH ＋ 10 μg/mL LH（control）,0,10,100,1000 ng/mL exogenous progesterone respectively into the in vitro maturation medium for bovine cumulus-oocyte complexes（COCs）.The germinal vesicle breakdown（GVBD） rate of each group after 8 h using acetic acid-orcein staining,and counted first polarbody（PbI） rate after 22 h were all analyzed.Finally,the blastocyst rate after 7-8 days of in vitro fertilization was also counted.The results showed that,GVBD（75.0%） and PbI（79.6%） rate of the control group were significantly higher than the other ones adding with exogenous progesterone（P＆lt;0.05）,and blastocyst rate of each group didn’ t show significant variation（P＆gt;0.05）.Therefore,it could be concluded that the exogenous progesterone could not play a role for improving the nuclear maturation of bovine oocytes,it also meant that exogenous progesterone alone could not take the same effect as endogenous progesterone in such kind of processes.