| 微孔板法测定功能性饮料中的维生素B_(12) |
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| 引用本文： | 张艳,王骏,舒艳丽,郑世超,胡梅. 微孔板法测定功能性饮料中的维生素B_(12)[J]. 山东农业科学, 2014, 0(4): 110-113 |
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| 作者姓名： | 张艳 王骏 舒艳丽 郑世超 胡梅 |
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| 作者单位： | 山东省产品质量检验研究院;山东省质量技术监督干部学校; |
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| 摘 要： | 通过样品处理比较试验、色素影响评价、回收率试验和重复性试验,得到了微孔板法测定功能性饮料中维生素B12的有效方法。经测定,该方法测定限可达到0.005μg/L,1.0、2.0、20.0μg/L三个梯度水平的加标回收率为94.4%~101.8%,实际样品测定中相对标准偏差为0.35%~4.34%(n=6),灵敏度和重复性很高,可用于测定功能性饮料中微量的维生素B12。
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| 关 键 词： | 微孔板法 测定 功能性饮料 维生素B12 Vitamin B12 |
Determination of Vitamin B12 in Energy Drinks by Microplate Method |
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| Zhang Yan,Wang Jun,Shu Yanli,Zheng Shichao,Hu Mei. Determination of Vitamin B12 in Energy Drinks by Microplate Method[J]. Shandong Agricultural Sciences, 2014, 0(4): 110-113 |
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| Authors: | Zhang Yan Wang Jun Shu Yanli Zheng Shichao Hu Mei |
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| Affiliation: | 1. Shandong Product Quality Inspection Research Institute, Jinan 250103, China ; 2. Shandong Cadre School of Quality Technology and Supervision, Jinan 250000, China) |
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| Abstract: | The optimal microplate method for determination of vitamin B 12 in energy drinks was confirmed through the tests on comparison of sample treatment methods , pigment impact assessment , recovery and repeat-ability.The detection limit of the method could reach 0.005μg/L.The recovery rate was 94.4%~101.8%at 1.0、2.0、20.0 μg/L of vitamin B12 gradient; the relative standard deviation in actual test was 0.35%~4.34%（n=6）.For the high sensitivity and reproducibility , this method could be used for the determination of light vitamin B12 in energy drinks. |
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| Keywords: | Microplate method Determination Energy drinks |
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