| 转AtNDPK2基因甘薯的耐盐性鉴定 |
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| 引用本文： | 侯夫云,赵兵,王庆美,解备涛,董顺旭,李爱贤,张海燕,张立明.转AtNDPK2基因甘薯的耐盐性鉴定[J].山东农业科学,2014(2):29-31. |
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| 作者姓名： | 侯夫云 赵兵 王庆美 解备涛 董顺旭 李爱贤 张海燕 张立明 |
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| 作者单位： | 山东省农业科学院作物研究所;济南市森林公园; |
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| 基金项目： | 国家科技支撑计划项目(2009BADA7B03);甘薯产业技术体系项目(CARS-11-B-06);山东省良种工程项目;济南市农业科技创新计划项目资助 |
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| 摘 要： | 以转AtNDPK2基因甘薯植株为试材,根据其生理指标及表型变化来鉴定转基因甘薯的耐盐性。室内鉴定结果表明:在100、150 mmol/L NaCl胁迫下,转基因株系JN1~JN6的根长显著高于对照。田间鉴定结果表明:在50 mmol/L NaCl胁迫下,转基因株系JN1~JN6生长正常,而对照10 d后出现黄叶及枯萎等症状;在100 mmol/L NaCl胁迫下,对照植株和JN2~JN6在处理4 d后出现枯萎,而JN1生长正常。
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| 关 键 词： | 甘薯 转基因 AtNDPK2 根长 耐盐性 AtNDPK2 |
Salt Tolerance Identification of Transgenic Sweet Potato Expressing AtNDPK2 Gene |
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| Hou Fuyun,Zhao Bing Wang Qingmei Xie Beitao,Dong ShunxuI,Li AixianI,Zhang HaiyanI,Zhang Liming.Salt Tolerance Identification of Transgenic Sweet Potato Expressing AtNDPK2 Gene[J].Shandong Agricultural Sciences,2014(2):29-31. |
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| Authors: | Hou Fuyun Zhao Bing Wang Qingmei Xie Beitao Dong ShunxuI Li AixianI Zhang HaiyanI Zhang Liming |
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| Institution: | 1. ( 1. Crop Research Institution, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2. Jinan Forest Park, Jinan 250100, China) |
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| Abstract: | The transgenic sweet potato plants expressing AtNDPK2 gene were used as materials to identi-fy their salt tolerance based on physiological indexes and phenotypic change .The results showed that the root length of transgenic lines （JN1, JN2, JN3, JN4, JN5 and JN6） was significantly longer than that of control under the stress of 100 mmol/L and 150 mmol/L NaCl .The transgenic plants grew normally , while the control leaves became yellow and withered after 10 days under the stress of 50 mmol/L NaCl.Under the stress of 100 mmol/L NaCl, JN1 grew normally after 4 days, while the others became withered . |
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| Keywords: | Sweet potato Transgene Root length Salt tolerance |
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