| 侵染丝瓜的黄瓜绿斑驳花叶病毒山东分离物分子鉴定 |
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| 引用本文： | 王云,辛志梅,赵黎明,竺晓平,王云祥. 侵染丝瓜的黄瓜绿斑驳花叶病毒山东分离物分子鉴定[J]. 山东农业科学, 2014, 0(8): 10-14 |
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| 作者姓名： | 王云 辛志梅 赵黎明 竺晓平 王云祥 |
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| 作者单位： | 昌乐县乔官镇农业综合服务中心;山东省农业科学院植物保护研究所;山东农业大学植物保护学院;安丘市兴安街道办公室; |
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| 基金项目： | 国家公益性行业(农业)科研专项(201303028);山东省自然科学基金(ZR2012CM032) |
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| 摘 要： | 2013年,在山东泰安采集到疑似感染黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)的丝瓜样品,利用该病毒外壳蛋白(Coat protein,CP)特异引物CG-CPF/CPR对样品进行扩增并测序。所得序列(GenBank登录号:KJ187042)经NCBI BLAST比对,与已登录的CGMMV序列的相似性均大于92.6%,确定为黄瓜绿斑驳花叶病毒。系统进化分析表明,CGMMV山东丝瓜分离物(CGMMV-SDLoofah)与辽宁分离物(CGMMV-LN)等大多数国内CGMMV分离物同聚为一个进化组,从而进一步确定了CGMMV山东丝瓜病毒分离物为黄瓜绿斑驳花叶病毒。这是首次报道黄瓜绿斑驳花叶病毒侵染丝瓜。
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| 关 键 词： | 丝瓜 黄瓜绿斑驳花叶病毒 外壳蛋白 分子鉴定 Cucumber green mottle mosaic virus (CGMMV) |
Molecular Identification of an Isolate of Cucumber Green Mottle Mosaic Virus Infecting Loofah in Shandong |
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| Affiliation: | Wang Yun, Xin Zhimei, Zhao Liming, Zhu Xiaoping, Wang Yunxiang ( 1. Qiaoguan Agricultural Extension Center of Changle County, Changle 262408, China;2. Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, China ;3. College of Plant Protection, Shandong Agricultural University, Taian 271018, China; 4. Xing＇an Street Office of Anqiu City, Anqiu 262100, China) |
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| Abstract: | An isolate of cucumber green mottle mosaic virus （ CGMMV） was obtained from loofah show-ing typical symptoms in Taian , Shandong Province , in 2013 .The nucleotide sequence of the coat protein （ CP） of this isolate （ CGMMV-SDLoofah ） was amplified and sequenced using specific primer pair CG -CPF/CPR.The sequence analysis showed the sequence of CGMMV -SDLoofah （ GenBank accession number：KJ187042 ） had 92 .6% of identity with other isolates of CGMMV in GenBank , so it was confirmed as the CGMMV.The phylogenic analysis results indicated that CGMMV -SDLoofah could be clustered together with Liaoning isolate （ CGMMV-LN） and most other CGMMV isolates from China , which further confirmed that the CGMMV-SDLoofah was an isolate of CGMMV .This was the first report of CGMMV infecting loofah in China . |
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| Keywords: | Loofah Coat protein Molecular identification |
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