水分吸收预处理提高菜用大豆种子活力

用40％PEG溶液、饱和湿度空气及多层滤纸控制吸胀预处理,能显著提高菜用大豆(Glycine max L.Merr.)种子的发芽势、发芽率和活力指数,以及抗低温吸胀和浸泡伤害的能力.在PEG溶液和多层滤纸条件下吸胀,明显减缓种子吸胀速率,特别是吸胀初期的速率.而且两者控制吸胀的效果相似.这三种处理显著地减少了种子浸泡液的电导率和紫外吸收物质量以及可溶性糖和氨基酸外渗量.种皮对阻止细胞内含物外渗有重要作用,但PEG等的修复作用主要发生在种皮内活的细胞组织.预处理对膜的修复有良好的效果,可是当种子劣变已伤及修复系统时,这种处理就无效.

Studies on vigor improvement of vegetable soybean seeds by controlled water absorption pretreatments.

When vegetable soybean (Glycine max L. Merr. ) seeds were treated with 409% PEG solution or water saturated atmosphere, the seed vigor was significantly improved, resulting in a higher germinative power, percentage germination and vigor index. Their resistance to cold injury during imbibition and soaking in water was also increased evidently. While the seeds were imbibed and germinated with several layers of filter paper, their effect on seed vigor was similar to the results above. When the seeds were imbibed with several layers of filter paper or in PEG solution, the water absorption rate was remarkably reduced, especially at the beginning of imbibition.The treatment also greatly reduced the leakage of electrolyte, O. D. 280nm substance, soluble sugar and amino acid. The results also showed that seed coat played an improtant role in preventing leakage from cells and the PEG repairing process mainly occurred in the living cells inside the seed coat. The pretreatments were effective in membrane repairing. But -when the seed deterioration did damage to the repairing system, the treatments were ineffective.