Tetrahydrofolate-dependent vanillate and syringateO-demethylation links tightly to one-carbon metabolic pathway associated with amino acid synthesis and DNA methylation in the lignin metabolism ofSphingomonas paucimobilis SYK-6 |
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Authors: | Tomonori Sonoki Yuichiro Otsuka Seiichiro Ikeda Eiji Masai Shinya Kajita Yoshihiro Katayama |
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Affiliation: | (1) Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, 184-8588 Tokyo, Japan;(2) Department of Bioengineering, Nagaoka University of Engineering, 940-2188 Nigata, Japan |
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Abstract: | Sphingomonas paucimobilis SYK-6 strain can degrade various lignin-related compounds. In the lignin metabolic pathway of this bacterium, vanillate and syringate are demethylated by the tetrahydrofolate (THF)-dependentO-demethylation system, which requires the enzymatic function of LigH. Upstream of theligH gene is the 5,10-methylene-THF reductase gene. Its gene product was essential for one-carbon metabolism involved in the amino acid synthesis and DNA methylation in all organisms. When themetF gene was inactivated in the genome of SYK-6, the resultant mutant, DLmetF, could not grow on vanillate and syringate as a sole carbon source. Furthermore, DLmetF showed significant accumulation of methyl-THF as a result of vanillate and syringateO-demethylation. We report here that THF-dependent vanillate and syringateO-demethylation links tightly to the one-carbon metabolic pathway that is associated with amino acid synthesis and DNA methylation, and the methyl group is the sole one-carbon source inS. paucimobilis SYK-6. |
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Keywords: | Lignin biodegradation Tetrahydrofolate One-carbon metabolism |
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