| 干、鲜品芭蕉根药材指纹图谱相似度聚类分析 |
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| 引用本文： | 许召林,许士娜,吴红梅,王祥培,靳凤云,钱海兵.干、鲜品芭蕉根药材指纹图谱相似度聚类分析[J].贵州农业科学,2012(8):45-48. |
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| 作者姓名： | 许召林 许士娜 吴红梅 王祥培 靳凤云 钱海兵 |
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| 作者单位： | 贵阳中医学院;山东医学高等学校附属医院;成都中医药大学 |
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| 基金项目： | 国家自然科学基金“贵州苗药芭蕉根抗糖尿病药效物质基础研究”(30860387);贵州省中药现代化公关计划项目“苗药芭蕉根有效组分筛选药物研究”[黔科合ZY(2012)3016] |
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| 摘 要： | 为了更好地利用、储藏芭蕉根,对干、鲜品芭蕉根指纹图谱相似度进行聚类分析,利用Dia-monsil C18色谱柱(250mm×4.6mm,5μm)乙腈-0.05%磷酸水梯度洗脱,流速1.0mL/min,检测波长310nm,测定了4种不同干、鲜品的芭蕉根药材指纹图谱,并对其相似度进行聚类分析。结果表明:鲜品芭蕉根与晾干品及冻干品芭蕉根指纹图谱相似度均有差异,但与烘干品芭蕉根较为相似。利用指纹图谱相似度及聚类分析方法可较全面地反映干、鲜品芭蕉根间的差异。
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| 关 键 词： | 芭蕉根 干品 鲜品 HPLC指纹图谱 聚类分析 |
Cluster Analysis of Fingerprint Similarity of Dry and Fresh Rhizoma Musae |
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| XU Zhao-lin,XU Shi-na,WU Hong-mei,WANG Xiang-pei,JIN Feng-yun,QIAN Hai-bing.Cluster Analysis of Fingerprint Similarity of Dry and Fresh Rhizoma Musae[J].Guizhou Agricultural Sciences,2012(8):45-48. |
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| Authors: | XU Zhao-lin XU Shi-na WU Hong-mei WANG Xiang-pei JIN Feng-yun QIAN Hai-bing |
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| Institution: | 1(1.Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 550002;2.Affiliated Hospital of Shandong Medical College,Linyi,Shandong 276001;3.Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan 611730,China) |
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| Abstract: | To preferably use and store Rhizome Musae and evaluate the fingerprint similarity of fresh and dry Rhizoma Musae with cluster analysis method.An analysis was performed on Diamonsil C18(250 mm×4.6 mm,5 μm).Acetonitrile and water containing 0.05% H3PO4 were used as mobile phases with gradient elution.The flow rate was set at 1.0 mL/min.The detection wavelength was set at 310 nm.The HPLC fingerprints of four different fresh and dry Rhizoma Musae were determined and the similarity was analyzed by cluster analysis.The results showed that There were apparent difference in HPLC fingerprint similarity except for stoving Rhizoma Musae.Fingerprint imilarity and cluster analysis could comprehensively show the difference of fresh and dry Rhizoma Musae. |
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| Keywords: | Rhizoma Musae dry rhizoma fresh rhizoma HPLC fingerprint cluster analysis |
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