| 耐低温乳酸菌的分离与优化培养 |
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| 引用本文： | 储徐建, 李长慧, 刘明灿, 李彩娟, 赵明霞. 耐低温乳酸菌的分离与优化培养[J]. 草业科学, 2014, 8(7): 1380-1388. DOI: DOI:10.11829/j.issn.1001-0629.2013-0536 |
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| 作者姓名： | 储徐建 李长慧 刘明灿 李彩娟 赵明霞 |
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| 作者单位： | 1.青海大学农牧学院,青海 西宁 810016 |
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| 摘 要： | 从青贮玉米(Zea mays)以及新鲜泡菜中分离乳酸菌,通过初筛和复筛获得1株产酸能力较强的耐低温乳酸菌L2;对其进行了形态学描述和生理生化鉴定,对照伯杰式手册确定其种属;采用Box-Behnken实验设计进行培养条件优化实验,确定了该菌的最佳发酵条件。结果表明,该菌的最佳发酵条件为接种量3.1%、初始pH6.4、培养温度18.4 ℃。
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| 关 键 词： | 耐低温乳酸菌 分离 优化培养 |
| 收稿时间： | 2013-09-13 |
Isolation and optimization of low temperature lactic acid bacteria |
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| Xu-jian CHU, Chang-hui LI, Ming-can LIU, Cai-juan LI, Ming-xia ZHAO. Isolation and optimization of low temperature lactic acid bacteria[J]. Pratacultural Science, 2014, 8(7): 1380-1388. DOI: DOI:10.11829/j.issn.1001-0629.2013-0536 |
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| Authors: | Xu-jian CHU Chang-hui LI Ming-can LIU Cai-juan LI Ming-xia ZHAO |
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| Affiliation: | 1.Agriculture and Animal Husbandry College of Qinghai University, Xining 810016, China |
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| Abstract: | One lactic acid bacteria strain L2 with low temperature resistant and more lactic acid production was isolated and selected from corn silage and fresh kimchi. The strain was identified with morphological, physiological and biochemical characterization according to the Bergey′s manual of systematic bacteriology. The culture conditions of this strain were optimized as 3.1% (v/v) inoculation amount, initial pH 6.4 and temperature of 18.4 ℃ with Box-Behnken Design. |
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| Keywords: | low temperature lactic acid bacteria isolation optimization of culture |
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