瓠瓜果实发育中的蛋白质SDS-PAGE分析

In seed plants, successful fruit set and subsequent development are dependent on pollination and fertilization since fertilization activates cell division and triggers fruit development by producing phytohormones especially auxins and GAs. Fruit set was very low in Lagenaria leucantha Rusby during early spring. We found that CPPU ［N-(chloro-4-pyridyl)-N′-phenyurea］, a new kind of cytokinin, was more effective than NAA and GA3 in inducing parthenocarpic fruit growth and in decreasing fruit abortion. In this paper, we examined the possible role of fertilization and CPPU in protein synthesis in developing fruit of Lagenaria leucantha by SDS-PAGE analysis.1　Materials and Methods1.1　Plant materials　　Seeds of Lagenaria leucantha Rusby (cv. Hangzhou long- gourd) were sown on 15 February 1999, and the seedlings were transplanted on 20 March. There were three treatments: Nonpollination; Hand pollination, and Nonpollination+50 mg/L CPPU. Unpollinated ovaries were prepared by bagging the female flowers one day before anthesis. The solution of CPPU was applied with a spray to ovaries of bagged female flowers at anthesis at the rate of 0.5 mL per ovary. Fruit length was recorded every three days. For assays of protein, the flesh of fruits at anthesis and 8 days after treatment was frozen in liquid nitrogen and stored at -20 ℃. 1.2　Extraction of proteins　　Half gram of fruit tissue was homogenized in 1 mL extract buffer containing 25 mmol/L DTT and 250 mmol/L Tris-HCl, pH6.8. The homogenate was centrifuged at 12 500 g for 20 min. Aliquot of the 250 μL supernatant was added into an eppendorf tube filled with 1 mL of 80% acetone, then frozen in -20 ℃ for 10 min. After thawing at 25 ℃, it was centrifuged at 10 000 g for 10 min, the supernatant was carefully discarded.……

SDS-PAGE Analysis of Protein Characteristics in Developing Fruit of Lagenaria leucantha

In seed plants, successful fruit set and subsequent development are dependent on pollination and fertilization since fertilization activates cell division and triggers fruit development by producing phytohormones especially auxins and GAs. Fruit set was very low in Lagenaria leucantha Rusby during early spring. We found that CPPU [N-(chloro-4-pyridyl)-N'-phenyurea], a new kind of cytokinin, was more effective than NAA and GA3 in inducing parthenocarpic fruit growth and in decreasing fruit abortion. In this paper, we examined the possible role of fertilization and CPPU in protein synthesis in developing fruit of Lagenaria leucantha by SDS-PAGE analysis.