砂梨变异的SRAP初步鉴定及亲缘关系分析

为了鉴定砂梨变异类型在DNA水平上是否发生遗传变异,以砂梨的两个变异单株及25个梨代表品种为试材,通过正交试验设计优化了SRAP扩增体系,并从88对SRAP引物中筛选出22对适宜的引物进行了PCR扩增,找出差异性片段,并应用系统聚类法,分析其与其它梨品种的亲缘关系。结果表明,砂梨适宜的SRAP体系为25μL反应体积中Mg2+2.4 mmol·L-1、dNTPs 0.1 mmol·L-1、Taq酶1.5 U、模板DNA 80 ng、引物0.18μmol·L-1;与对照品种‘清香’相比,‘02-1’有7条差异性片段,‘02-2’有13条差异性片段,说明其均在DNA水平上存在差异;聚类分析表明,‘02-1’首先与‘清香’聚为一类,‘02-2’先与‘新世纪’和‘黄花’聚类,再与‘清香’聚类,因此,推断‘02-1’可能为‘清香’的营养系变异,‘02-2’可能为‘清香’的姊妹系。

Primarily identification of pear variants and analysis of their genetic relationship by SRAP markers

To identify wether there exit genetic variation happened in Japanese pear(Pyrus pyrifolia Nakai),27 accessions including the two variants '02-1' and '02-2' and other twenty-five cultivars were studied by method of sequence-related amplified polymorphism(SRAP) markers.Firstly,the SRAP-PCR reaction system was opti-mized with orthogonal design.22 pairs of suitable primers screened out from 88 ones were used to detect the dif-ferential fragment among the pear cultivars.The genetic relationships between accessio...