传统中药材麦冬的DNA条形码鉴定

该文分析了DNA条形码序列(ITS、psb A-trnH、matK、rbcL和trn L-F)对采自10个不同产区的麦冬进行PCR扩增及测序。结果表明,ITS序列变异位点为11 bp且较稳定。叶绿体序列的变异位点较低。本研究构建了ITS及联合叶绿体片段与ITS的系统发育树,4个叶绿体片段各自构建的系统发育树结果不理想,以ITS构建的系统发育树为主。广西桂林、贵州贵定与浙江余杭聚为一支;四川什都、四川珙县与广东肇庆聚为一支;云南麻栗坡与云南石林聚为一支;江西庐山与湖南桑植聚为另一分支,位于发育树基部;以上分支均得到G+C含量和遗传距离的支持,种内具有较近的亲缘关系。ITS序列具有稳定的变异位点和鉴定位点,能够准确的鉴定不同产地的麦冬,基于ITS构建的系统发育树能够较好的区分其亲缘关系。

DNA Barcoding Technique to Identify Traditional Chinese Medicinal Materials Ophiopogon japoni-cas(L.f.)Ker-Gawl

DNA barcoding sequences(ITS,psbA-trnH,matK,rbcL,and trnL-F)were used to study O.japonicus from 10 different regions by PCR technique. The results showed that sequence variable sites of ITS were 11 bp and stable. Variable sites of chloroplast were low.In this study,the ITS and ITS+chloroplast phylogenetic trees were constructed, the phylogenetic trees based on chloroplast fragments were poor.ITS phylogenetic tree was dominant.O.japonicas from Guilin in Guangxi,Guiding in Guangzhou and Yuhang in Zhejiang comprised one closely clade;from Shendu in Sichuan,Gongxian in Sichuan and Zhaoqing in Guangzhou formed a closer clade;from Malipo in Yunnan and Shilin in Yunnan grouped into one closely clade;and from Lushan in Jiangxi and Sangzhi in Hunan formed another closely clade.All above clades were supported by G+C contents and genetic distances,and had closely relationships. The ITS sequence had stable variable sites and informative sites,and could accurately indentify O.japonicas from 10 dif?ferent regions. The ITS phylogenetic tree could provide reliable genetic relationships.