意大利生菜组织培养体系的建立

目的]建立意大利生菜组织培养体系。方法]以意大利生菜(Lactuca sativa L.var.ramosa Hort.)种子为试验材料,用不同有效氯含量(0、0.5%、1.0%、2.0%、4.0%)的漂白水灭菌,于MS培养基上培养无菌苗。取4~5 d苗龄的无菌幼苗子叶,在添加不同浓度激素的MS培养基上诱导愈伤组织,并进一步诱导生芽、生根,筛选最佳诱导激素配比及激素浓度。结果]意大利生菜种子经有效氯含量1.0%的漂白水处理,于MS培养基上培养可获得意大利生菜无菌苗。无菌幼苗子叶在MS+0.5 mg/L 6-BA+0.20 mg/L NAA+30 g/L蔗糖+7 g/L琼脂的培养基上,可以得到理想的意大利生菜愈伤组织;在MS+0.25 mg/L 6-BA+0.20 mg/L NAA+30 g/L蔗糖+7 g/L琼脂的培养基上,最有利于意大利生菜愈伤组织不定芽的发生且对后期生根有良好的诱导作用。结论]配合使用一定浓度的6-BA和NAA可有效建立意大利生菜组织培养再生体系,为进一步建立意大利生菜遗传转化体系奠定基础。

Establishment of Tissue Culture System of Lactuca sativa L.var.ramosa Hort.

Objective] To establish the tissue culture system for Lactuca sativa L.var.ramosa Hort.Method] Lactuca sativa L.var.ramosa Hort.seeds were disposed with different concentration javelle (0,0.5 ％,1.0％,2.0％,4.0％) for sterilization,and aseptic seedlings were cultured on MS medium.Taking cotyledons from the 4-5 days of seedlings as explants,the ideal sorts and concentrations of plant hormones were tested for induction of calluses,buds and roots seperately.Result] Lactuca sativa L.var.ramosa Hort.seeds were disposed with 1.0％ available chlorine javelle and aseptic seedlings were gotten on MS culture medium.Taking cotyledons from the 4-5 days of seedlings as explants,the ideal calluses were induced and generated on the medium of MS medium supplied with 0.5 mg/L 6-BA,0.20 mg/L NAA,30 g/L sucrose and 7 g/L agar.The most in favor medium for bud differentiation from calluses were MS medium supplied with 0.25 mg/L 6-BA,0.20 mg/L NAA,30 g/L sucrose and 7 g/L agar,which was also beneficial for rooting subsequently.Conclusion] The appropriate concentration of 6-BA and NAA was effective for Lactuca sativa tissue culture,and lay a foundation for establishing geetic transformation system of Lactuca Sative L.var.ramosa Hort.