天麻共生萌发菌栽培种生产工艺条件优化 |
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引用本文: | 程立君,段相程,胡志芳,李世辉,赵艳娟,师睿.天麻共生萌发菌栽培种生产工艺条件优化[J].安徽农学通报,2016,22(6). |
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作者姓名: | 程立君 段相程 胡志芳 李世辉 赵艳娟 师睿 |
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作者单位: | 昭通学院化学与生命科学学院,云南昭通,657000;昭通苹果研究院,云南昭通,657000 |
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基金项目: | 云南省高校昭通高原特色农产品工程研究中心项目(16002)。 |
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摘 要: | 经试验对天麻共生萌发菌MY-001的栽培种生产的工艺条件进行了优化,结果显示,既能降低生产成本又能提高生产效率的液体发酵条件为:液体菌种培养器的气压为0.020MPa,发酵液主要成分中红糖、麦麸和蛋白胨的最佳比例为红糖10g/L、麦麸15g/L、蛋白胨1g/L,萌发菌菌丝生物量随时间的变化符合逻辑斯蒂增长模型,其观测值与逻辑斯蒂方程拟合值的拟合度较高(R2=97.7%),发酵培养144h后生物量趋于稳定,栽培种基质最合适的比例为壳斗科植物树叶60%、阔叶树木屑30%和麦麸10%。
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关 键 词: | 天麻 萌发菌 液体发酵 工艺优化 |
Optimizing the Gastrodia elata Seed-germinating Strains Production Process |
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Abstract: | The best production process conditions for Gastrodia elata seed-germinating strain MY-001 were opti?mized. The results showed that the best liquid fermentation conditions were pressure of 0.020MPa,ermented liquid of brown sugar 10g/L,wheat bran 15g/L,peptone 1g/L. The number increasing ofhypha biomass over time accords with the Logisticgrowth model,the observations fit with the logistic equation fitting thehigher value(R2=97.7%),bio?mass tends to be stable after fermentation 144h. The optimum cultivation of the substrate were determined fagaceae plant leaves60%,broadleaf sawdust 30%,and wheat bran 10%. |
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Keywords: | Gastrodia elata Germinating strain Liquid fermentation Process optimization |
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