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癸培养基在小麦花药脱分化培养中的应用研究
引用本文:海燕,康明辉,赵永英,何宁,张丹. 癸培养基在小麦花药脱分化培养中的应用研究[J]. 河南农业科学, 2010, 0(9)
作者姓名:海燕  康明辉  赵永英  何宁  张丹
作者单位:河南省农作物新品种重点实验室,河南,郑州,450002
摘    要:采用癸、C172种培养基进行了9 a的小麦花药培养脱分化试验,共计接种杂交组合505个。结果表明,癸培养基愈伤组织平均诱导率为5.36%,C17培养基为4.38%,癸培养基比C17培养基的诱导率提高了22.4%,且愈伤组织诱导率较稳定,二者差异达到显著水平。试验表明,癸培养基是适宜于黄淮麦区的小麦花药培养的脱分化培养基。

关 键 词:癸培养基  小麦  花药培养  愈伤组织

Application of Gui Medium in Wheat Dedifferentiation of Anther Culture
HAI Yan,KANG Ming-hui,ZHAO Yong-ying,HE Ning,ZHANG Dan. Application of Gui Medium in Wheat Dedifferentiation of Anther Culture[J]. Journal of Henan Agricultural Sciences, 2010, 0(9)
Authors:HAI Yan  KANG Ming-hui  ZHAO Yong-ying  HE Ning  ZHANG Dan
Abstract:The dedifferentiation experiment of wheat anther culture was conducted for nine years with Gui and C17 medium respectively,and in total 505 hybrids were inoculated.The result showed that the average inductivity of callus in 9 years was 5.36% in Gui medium,22.4% higher and more stable than in C17 medium(4.38%),and the difference being significant.It suggested that Gui medium is suitable for the dedifferentiation of wheat anther culture in Huanghuai wheat area.
Keywords:Gui medium  Wheat  Anther culture  Callus
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