利用SRAP、SSR和AFLP 标记构建甘蓝型油菜遗传连锁图谱

 【目的】建立甘蓝型油菜的高质量遗传图谱，为在分子水平上研究复杂性状打下基础并提供保证。【方法】以甘蓝型油菜自交不亲和系“SI-1300” 及其恢复系“Eagle”组配得到的184个F2单株为群体，利用SRAP、SSR和AFLP标记技术构建甘蓝型油菜遗传图谱。【结果】该图谱共包含21个连锁群，涉及137个SRAP标记、143个SSR标记和118个AFLP标记。图谱总长1 949.8 cM，标记间平均图距为4.9 cM。以SSR标记为锚定标记，该图谱与国际标准图谱进行了初步对应。研究共发现了8个偏分离区域。【结论】根据对标记分布的均匀程度，图谱覆盖程度以及标记密度等方面的分析，本研究构建的甘蓝型油菜分子遗传图谱质量较高，并且SRAP标记可能是比AFLP标记更适合于图谱构建的标记体系。

Construction of a Linkage Map Using SRAP, SSR and AFLP Markers in Brassica napus L.

A genetic linkage map of Brassica napus was constructed using 143 SSR, 118 AFLP, and 137 SRAP markers based on an F2 population of 184 individuals, which was developed by crossing “SI-1300” and “Eagle”. These markers were aligned in 21 linkage groups (LGs), and the map covered 1949.8 cM with an average marker interval of 4.9 cM. The public microsatellites (SSR markers) contributed to establish the links between the map of Parkin et al. and ours. Our results showed that most of LGs were aligned with the standard map except LG 20 and LG 21，and the distribution of markers on all LGs were uniform except N7 based on the analysis of Kolmogorov-Smirnov. However, the distorted markers were mainly cluster in LGs, and eight segregation distortion regions on seven LGs were detected. In addition, the results showed that SRAP markers may be a better type of markers than AFLP in map construction. In conclusion, according to the analysis of the marker distribution on LG, the map coverage and the marker density, the linkage map constructed in this study was of enough quality .Threrefore, this map will be a fine platform for the location of QTLs, studies on heterosis, and using the information of others.