Comparison of insecticide resistance in a North American field population and a laboratory colony of potato tuberworm (Lepidoptera: Gelechiidae)

Potato tuberworm, Phthorimaea operculella Zeller, has become a serious threat to potato production in the Columbia Basin region of Oregon and Washington and in 2005 was found in Idaho. Despite intensive insecticide application, severe defoliation has occurred in recent years indicating insensitivity of potato tuberworms (PTW) to insecticides. In this study, dose responses of potato tuberworms from a Columbia Basin field population (CBFP) and a laboratory colony from Colorado (COLC) to three commonly used field crop insecticides (esfenvalerate, methamidophos and fipronil) were tested. The COLC was included in this study as a standard susceptible colony but despite being reared in isolation for >30 years, it was resistant to field-labeled rates of all three insecticides used in this study. The CBFP was also resistant to esfenvalerate and fipronil at field rates but was relatively susceptible (40% mortality) to methamidophos. At 10× field rate of fipronil, COLC was very susceptible (>90% mortality) but CBFP was not significantly affected. CBFP was also more resistant to esfenvalerate than COLC. None of the COLC survived a 500× field rate of esfenvalerate; the same dose killed only 10% of the CBFP. Although further tests are required to more broadly assess the levels and types of insecticide resistance in these populations, our findings indicated that the laboratory colony was susceptible to fipronil at dosages somewhat greater than labeled field rates and that the field population was susceptible to methamidophos. Although COLC was moderately insensitive to esfenvalerate and methamidophos, this laboratory colony was still more broadly susceptible to field rates than the CBFP.