| 人工合成免疫刺激CpG DNA联合鸡新城疫疫苗对SPF鸡免疫应答的影响 |
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| 引用本文： | 田兴山,张玲华,周风珍. 人工合成免疫刺激CpG DNA联合鸡新城疫疫苗对SPF鸡免疫应答的影响[J]. 中国兽医学报, 2006, 26(1): 14-16 |
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| 作者姓名： | 田兴山 张玲华 周风珍 |
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| 作者单位： | 1. 广东省农业科学院,农业生物技术研究所,广东,广州,510640
2. 广东省农业科学院,农业生物技术研究所,广东,广州,510640;华南农业大学生命科学院,广东,广州,510642 |
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| 基金项目： | 广东省广州市科技攻关项目;广东省农业科技攻关项目 |
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| 摘 要： | 以鸡新城疫病毒为模式病毒，分别以不同浓度人工合成的免疫刺激序列CpG DNA联合鸡新城疫低毒力活疫苗免疫SPF新生小鸡，通过ELISA法检测鸡血清中抗-NDV抗体水平，并用MTT法检测淋巴细胞白介素-2诱生活性以及淋巴细胞增殖指数（SI），结果显示：不同浓度的CpGDNA均能显著提高仔鸡的特异性抗体滴度、淋巴细胞白介素-2诱生活性以及淋巴细胞增殖反应，证明CpGDNA能显著增强鸡对常规疫苗的免疫应答能力。
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| 关 键 词： | 鸡新城疫低毒力活疫苗 免疫应答 |
| 文章编号： | 1005-4545(2006)01-0014-03 |
| 修稿时间： | 2004-02-17 |
Effect of Different Concentration of CpG Motifs on Immune Responses of SPF Chicken |
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| TIAN Xing-shan,ZHANG Ling-hua,ZHOU Feng-zhen. Effect of Different Concentration of CpG Motifs on Immune Responses of SPF Chicken[J]. Chinese Journal of Veterinary Science, 2006, 26(1): 14-16 |
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| Authors: | TIAN Xing-shan ZHANG Ling-hua ZHOU Feng-zhen |
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| Abstract: | The newborn chicken were immunized by the different concentration of CpG motifs and Newcastle disease mild living vaccine,the titre of specific antibodies to Newcastle disease mild living vaccine were measured by ELISA,the proliferation of lymphocytes and induced IL-2 activities were detected by MTT.The results showed that the above-mentioned immune responses of the newborn chicken to different concentration of CpG motifs were significantly stronger than routine immunities. |
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| Keywords: | CpG |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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