菊花品种资源遗传多样性的AFLP分析

以AFLP—银染分子标记技术, 对45个菊花品种进行了遗传多样性和亲缘关系分析。选用10个多态性高、分辨力强的E + 3 /M + 3引物组合分别对供试材料的基因组DNA进行扩增, 共获得486条清晰可辨的条带, 其中多态性带451条, 平均每个引物组合可检测出4511个多态性位点, 多态性位点百分率高达92.80% , 这表明供试品种资源在DNA水平上酶切位点的分布存在广泛的变异。应用DPS软件计算供试品种遗传距离界于0.36000～0.86237之间, 平均为0.611185; UPGMA分析将45份资源分成6个类群,从相异性系数分析了各品种资源间的亲缘关系。

Genetic Diversity of Chrysanthemum Cultivars Revealed by AFLP Analysis

The genetic diversity and relationship of 45 chrysanthemum cultivars were analyzed by AFLP (Amplified Fragment Length Polymorphism)-silver staining protocol. AFLP fingerprinting of 45 chrysanthemum cultivars with ten pairs of E 3/M 3 primers revealed a total number of 486 unambiguous bands, of which 451 were polymorphic and 45.1 polymorphic bands per pair of primer were produced on average. The polymorphism frequency was 92.80%. This result showed the abundant diversities of enzyme digestion sites among used chrysanthemum cultivars. As analyzed by DPSv3.01, the Nei's genetic distance of 45 chrysanthemum cultivars ranged from 0.36000 to 0.86237, and the average distance was 0.611185.These chrysanthemum cultivars were divided into six groups by UPGMA(unweighted pair group method with arithmetic average) based on Nei's genetic distances. The genetic relationship of 45 chrysanthemum cultivars was analyzed using the different coefficient.