Identification of cultivars of pasture legumes (Centrosema macrocarpum,C. pubescens and C. sp.n.) by acid polyacrylamide gel electrophoresis of cotyledons storage proteins |
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Authors: | A Hussain H Ramirez W M Roca W Bushuk |
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Institution: | (1) Department of Food Science, University of Manitoba, R3T 2N2 Winnipeg, Manitoba, Canada;(2) Biotechnology Research Unit, Centro Internacional de Agricultura Tropical-CIAT, Apartado Aéreo 6713, Cali, Colombia |
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Abstract: | Summary An electrophoretic procedure was developed for storage proteins which can discriminate cultivars of forage legumes, Centrosema macrocarpum, C. pubescens and C. sp.n. Proteins extracted from cotyledons were separated in 10% polyacrylamide gel containing 10% sucrose. Electrophoretic patterns are presented to illustrate the results that can be obtained with the procedure described. Cultivars of all three species were distinguishable based upon the variation in their acid soluble seed proteins.Joint publiccation of the Department of Food Science, University of Manitoba (No 101) and Centro Internacional de Agricultura Tropical-CIAT. |
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Keywords: | Centrosema macrocarpum Centrosema pubescens cultivar identification Acid PAGE Storage proteins pasture legume |
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