猪肺泡巨噬细胞感染PRRSV进程中差异表达基因及基因功能富集分析

猪呼吸和繁殖综合症病毒（porcine reproductive and respiratory syndrome virus,PRRSV）感染猪后会引起妊娠母猪严重繁殖障碍和仔猪呼吸困难及高死亡率,给养猪产业造成了巨大经济损失,因此挖掘PRRSV感染猪肺泡巨噬细胞进程中差异表达的基因及机理有重要意义。本研究对来自GEO数据库的PRRSV感染猪肺泡巨噬细胞sage表达谱数据GSE10346进行后续挖掘。先用sage软件处理,经sagemap注释得到上调已知基因49个,下调已知基因41个。利用AgBase网站上的GORetriever工具和GOSlim Viewer工具进行细胞组分（cellular component）、分子功能（molecular function）及参与的生物过程（biological process）分类后发现有4个基因与病毒的复制相关：其中IL8已有报道认为与抗PRRSV相关;TNF-α能够抑制PRRSV复制;PPIA可能与GP5蛋白胞外区中和决定簇与抗体发生作用的24位脯氨酸有关;ICAM-1在嗜中性粒细胞的产生中起关键作用,与病毒吸附相关。这4个基因与PRRSV的致病机制相关,可望用于抗PRRSV研究。进一步利用David在线工具进行基因功能富集分析后,发现几个免疫通路,如趋化因子通路,Toll-like受体信号通路和NOD-like受体信号通路具有检验显著性,这些免疫通路在PRRSV感染过程中起着一定作用,有望成为抗PRRSV的靶通路。

Differentially Expressed Genes and Gene Function Enrichment Analysis in the Process of Porcine Alveolar Macrophages Infected with PRRSV

Porcine reproductive and respiratory syndrome virus（PRRSV） can cause serious reproductive failure in pregnant sows,breathing difficulties and high mortality rates in piglets,and has caused tremendous economic losses in the swine industry,so mining differentially expressed genes and mechanism in the process of PRRSV infection of porcine alveolar macrophages are important.Sage expression data GSE10346 of porcine alveolar macrophage infected with PRRSV from GEO database were used for subsequent excavation in the present study.We obtained 49 up-regulated known genes and 41 down-regulated known genes after processing with the sage software and annotating with sagemap.Four genes were found associated with viral replication by using AgBase site GORetriever tools and GOSlimViewer tools for differentially expressed genes classification according to cellular component,molecular function and biological process.Thereinto,IL8 is correlated with anti-PRRSV and TNF-α can inhibit PRRSV replication.The presence of a proline in position 24 is required for a neutralization epitope in the N-terminal ectodomain of the GP5 protein interacts with antibodies,which may be associated with PPI-A.ICAM-1,associated with the virion attachment,play a key role in the production of neutrophils.The four genes associated with the pathogenesis of PRRSV may be used for PRRSV resistance.It was found several immune pathways,such as the chemokine pathway,Toll-like receptor signaling pathway and NOD-like receptor signaling pathway,which play a role in the process of PRRSV infection,were significant pathway by gene functional enrichment analysis,and are expected to be the target pathways against PRRSV.