小峰熊蜂工蜂蛹期发育蛋白质组分析

本文采用双向凝胶电泳法对小峰熊蜂工蜂蛹期的3个发育期进行蛋白质组研究，结果表明小峰熊蜂工蜂蛹期的白眼期（A期）、褐眼期（B期）和黑眼期（C期）分别检测到77、81和59个蛋白点，特有蛋白分别为7个、6个和1个，共有蛋白为48个，A期到B期显著上调的蛋白有9个，显著下调的蛋白有3个，B期到C期显著上调的蛋白有8个，显著下调的蛋白有3个，A期到C期显著上调的蛋白有15个，显著下调的蛋白有2个。研究还发现有17个蛋白是在A期和B期表达而在C期关闭；有2个蛋白是在A期表达，B期关闭，在C期又表达。本研究初步揭示了小峰熊蜂工蜂从蛹期发育到成蜂过程中，不仅需要一些保守蛋白来调控，而目．还需要一此特异蛋白。

Proteome Analysis of the Pupae Development ofBombus hypocrite workers

In this paper, we investigated the proteome of the pupal development of B. hypocrite worker by using two-dimensional gel electrophoresis. The results showed that 77, 81 and 59 proteins were detected in white-eyed pupal stage （A stage）, brown-eyed pupal stage （B stage） and dark-eyed pupal stage （C stage） respectively, 7 proteins, 6 proteins and 1 protein were specific to A stage, B stage and C stage respectively. Meanwhile, 48 proteins were presented in three stages, among which 9 proteins were significantly up-expressed and 3 proteins were significantly down-expressed from A stage to B stage, 8 proteins were remarkably up-expressed and 3 proteins were remarkably down-expressed from B stage to C stage, 15 proteins were obviously up-expressed and 2 proteins were obviously down-expressed from A stage to C stage. Besides, we still found that 17 proteins expressed both in A stage and B stage, but silenced in C stage, and 2 proteins expressed in A stage, but silenced in B stage, then expressed in C stage. It can therefore be concluded that the development from pupae to adult need not only house keeping proteins, but also specific ones.