| 利用组织培养技术提取甘草黄酮 |
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| 引用本文： | 王彦芹,焦培培,张莉,康少峰,严建龙,李彬.利用组织培养技术提取甘草黄酮[J].广西农业生物科学,2010(6):1111-1117. |
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| 作者姓名： | 王彦芹 焦培培 张莉 康少峰 严建龙 李彬 |
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| 作者单位： | [1]塔里木大学生命科学学院,阿拉尔新疆843300 [2]新疆生产建设兵团塔里木盆地生物资源保护与利用重点实验室,阿拉尔新疆843300 |
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| 基金项目： | 塔里木大学大学生课外创新项目（TDKWZKCX08005）; 兵团青年科技创新资金专项（2010JC48）共同资助 |
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| 摘 要： | 本研究筛选了两种能快速诱导甘草疯长型愈伤组织的培养基MS5和MS7,用微波法提取甘草愈伤组织和野生甘草根中总黄酮。测定结果显示：三年生野生型胀果甘草根中总黄酮的含量约为6.02%,培养3~4周的胀果甘草愈伤组织中总黄酮的含量约为1.1%。结果表明：组织培养生产黄酮在生产效率、工厂化生产、避免季节限制、保护野生甘草资源及实际应用中更具优势。本研究可为从甘草中提取总黄酮提供一条可借鉴的途径。
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| 关 键 词： | 组织培养 疯长型愈伤组织 黄酮产量 |
Study on Extraction of Flavonoids Contents from Glycyrrhiza Callus by Plant Tissue Culture |
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| Wang Yanqin,Jiao Peipei,Zhang Li,Kang Shaofeng,Yan Jianlong,Li Bin.Study on Extraction of Flavonoids Contents from Glycyrrhiza Callus by Plant Tissue Culture[J].Journal of Guangxi Agricultural and Biological Science,2010(6):1111-1117. |
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| Authors: | Wang Yanqin Jiao Peipei Zhang Li Kang Shaofeng Yan Jianlong Li Bin |
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| Institution: | 1 College of Life Science of Tarim University, Alar, Xinjiang, 843300; 2 Key Laboratory of Protection ＆ Utilization of Biological Resource in Tarim Basin of Xinjiang Production and Construction Groups, Alar, Xinjiang 843300 |
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| Abstract: | The best culture medium for callus induction was selected out： MS5 and MS7. Glycyrrhiza flavonoids was seprated using of microwaves. The result is flavonoids is about 1.1% in glycyrrhiza callus, and 6.02% in roots of wild glycyrrhiza. The results showed that plant tissue culture is better than wild glycyrrhiza at flavonoids production about productivity, avoiding seasonal limited, and protecting resources of wild glycyrrhiza. |
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| Keywords: | Plant tissue culture Over growth callus Flavonoids production |
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