红颊草莓离体培养及其增殖过程中可溶性蛋白研究

本文以红颊草莓为试材,对红颊草莓茎段进行组织培养,筛选出离体快繁最适的培养基条件,并诱导再生植株,也初步探索了培养物增殖过程中可溶性蛋白的动态变化。结果表明：红颊草莓适宜的诱导分化培养基为MS＋6-BA0.5mg/mL＋IAA0.25mg/mL,不定芽分化率可达100%;继代增殖最适宜培养基为MS＋6-BA0.25mg/L＋KT0.25mg/L＋IAA0.05mg/L,增殖倍数达到6.44;最佳生根培养基为1/2MS＋IAA0.05～0.1mg/L,生根数为5～6条,平均根长2cm左右,且生长健壮;最佳移栽基质为河沙,移栽成活率达88.9%。对红颊草莓增殖过程中的可溶性蛋白研究结果表明：可溶性蛋白含量在第10天、第30天呈现明显的两个高峰,SDS-PAGE电泳结果显示16.7kD、15.6kD和14.4kD的蛋白是草莓生长所必须的,而分子量为26.2kD和23.7kD的蛋白则只在继代增殖旺盛时期出现,为增殖时的特异蛋白组分。本研究结果为红颊草莓快繁育苗提供了一条高效途径,也为进一步研究草莓离体培养过程中体内生理变化的分子机理提供了有意义的参考。

Studies of Hongjia Strawberry in vitro Cultivation and Its Soluble Protein of Proliferation

In this paper,we employed the Hongjia strawberry as the test materials to study the tissue culture of Hongjia strawberry creeping stems,to select the best medium condition of rapid propagation of strawberry in vitro,and then induced plant regeneration,and finally,we also primarily explored the dynamic changes of soluble proteins of cultivation in proliferation.The results showed that the suitable differentiation medium was MS＋6-BA 0.5 mg/L＋IAA 0.25 mg/L,and its differentiation rate of adventitious shoots was up to 100%;The optimum subculture proliferation medium was MS＋6-BA 0.25 mg/L＋KT 0.25 mg/L＋IAA 0.05 mg/L,the proliferation of multiple was 6.44;The best rooting medium was 1/2MS＋IAA 0.05~0.1 mg/L,the average number of roots was 5~6,the average root length was 2 cm and they grew robustly;on the other hand,we also found that the best transplant matrix was river sand and its ransplant survival rate would reach 88.9%.The results in soluble proteins of Hongjia strawberry proliferation demonstrated that there are two obvious peaks in the 10th day and 30th day in terms of soluble protein content and the results of SDS-PAGE displayed that the protein of 16.7 kD,15.6 kD and 14.4 kD was essential for the growth of strawberry,however,the protein of 26.2 kD and 23.7 kD was only appeared in the period of proliferation,that is the specific proteins for proliferation.The results of this investigation would provide an highly efficient way for rapid propagation of Hongjia strawberry and a meaningful reference for further investi gating the molecular mechanisms of physiological changes of Hongjia strawberry in vitro cultivation.