含氟核苷在生物大分子结构机理研究中的应用进展

氟原子半径小、电负性强、具有与（-OH）官能团类似的氢键形成能力,是＂超级卤素＂。生物大分子核酸中引入氟原子,会使得氟原子附近的原子电子云密度、碱性及酸性发生明显变化,从而导致整个核酸分子构象由于偶极相互作用发生变化。本文主要综述最近几年含氟核苷在蛋白质与核酸的相互作用及其动力学机制、DNA和RNA的二级结构等生物大分子结构机理研究中的应用,同时,进一步阐述了利用含氟核苷,结合19F-NMR核磁共振波谱技术,研究核酸与蛋白质之间特异性识别的结构与动态机制及核酸二级结构的研究进展,以期氟化学在我国化学和生物学领域得到更为广泛的应用。

Application of Fluoro-Contained Nucleotide in Biological Molecular Structural Mechanism

In all the elements,fluorine has small atomic radius,strongest electronegativity,and similar hydrogen bond forming ability with hydroxyl group （-OH）,it is so called ＂super-halogen＂.The introduction of fluorine atoms to nucleic acids of biological macromolecules will change electron density of neighboring atoms,alkalinity and acidity significantly,resulting in the DNA conformation changes as the dipole-dipole interaction.In this paper,we mainly reviews the recent application of fluorinated nucleoside in the mechanism and structure of biological macromolecules on the interaction between protein and nucleic acid and its dynamics mechanism.The secondary structure of DNA and RNA and so on.On the other hand,we further describes the recent progress of fluorinated nucleosides in studying the specific recognition of nucleic acids and proteins,the structure and dynamics of mechanisms and nucleic acid secondary structure by utilizing fluorinated nucleoside with combination of 19F-NMR nuclear magnetic resonance spectroscopy technique.We expect that it would be applied extensively in the field of fluorine chemistry of chemical biology.