象草腋芽外植体消毒方法的筛选

为了解决象草组织培养中外植体污染问题,提高象草组织培养的成功率。本研究运用正交设计实验设置自来水冲洗、0.1%升汞浸泡和2%次氯酸钠浸泡3因子的不同消毒组合,通过直观分析和方差分析对象草腋芽外植体消毒灭菌结果进行分析。结果表明,象草腋芽外植体消毒的最佳组合为:自来水冲洗60min,0.1%升汞浸泡25min,2%次氯酸钠浸泡20min。该组合不仅能使污染率降低到13.33%,同时又在外植体的承受范围之内,没有对外植体造成伤害,本文结果将为象草的离体再生培养提供技术参考。

Screening of Explant Disinfection Method for Pennisetum purpureum Axillary Bud

In order to account for the matter of explants pollution of Pennisentum purpureum during tissue culture and improve its cultivar succece rate. In this research, we set up different disinfection combinations of wash tap water, immersing in 0.1% HgCl2, and immersing in 2.0% sodium hypochlorite solution three factors to analyze the sterilization results of axillary bud explants of Pennisentum purpureum by using orthogonal design experiments based on intuitive construe and analysis of variance. The results indi...