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不同温度贮藏后山豆根种子酶活性与发芽率相关性研究
引用本文:覃柳燕,唐美琼,梁莹,李林轩,缪剑华.不同温度贮藏后山豆根种子酶活性与发芽率相关性研究[J].种子,2011,30(11).
作者姓名:覃柳燕  唐美琼  梁莹  李林轩  缪剑华
作者单位:广西壮族自治区药用植物园,南宁,530023
基金项目:国家发展改革委经济运行局中药材扶持资金项目
摘    要:运用氮蓝四唑(NBT)法、愈创木酚-过氧化氢比色法和紫外吸收法,分别测定不同温度贮藏及新采收的山豆根种子的SOD、POD、CAT活性,并测定发芽率,探讨两者的相关性.结果表明,山豆根种子的SOD、POD、CAT酶活性与发芽率均随贮藏时间的延长逐渐减弱,SOD、POD、CAT三种抗氧化酶活性与山豆根种子生命力的丧失有关,但不是影响山豆根种子生命力最关键的酶类.

关 键 词:山豆根  超氧化歧化酶  过氧化物酶  过氧化氢酶  发芽率

Study on the Relationship Between Antioxidant Enzymes Activities and Germination Rate of Sophora tonkinensis Gagnep Seed Storaged at Different Temperatures
QIN Liu-yan,TANG Mei-qiong,LIANG Ying,LI Lin-xuan,MIAO Jian-hua.Study on the Relationship Between Antioxidant Enzymes Activities and Germination Rate of Sophora tonkinensis Gagnep Seed Storaged at Different Temperatures[J].Seed,2011,30(11).
Authors:QIN Liu-yan  TANG Mei-qiong  LIANG Ying  LI Lin-xuan  MIAO Jian-hua
Institution:QIN Liu-yan,TANG Mei-qiong,LIANG Ying,LI Lin-xuan,MIAO Jian-hua(Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China)
Abstract:This paper studied the relations between antioxidant enzymes(SOD,POD and CAT) activities and germination rate of Sophora tonkinensis Gagnep seed storaged at different temperatures by NBT,Methoxyphenol-H2O2 colorimetry,Ultraviolet absorption and traditional germination technique.Results showed that SOD,POD and CAT activities and germination rate decreased with the store time prolonged.The activities of SOD,POD and CAT related with seed vigor of Sophora tonkinensis Gagnep disappeared or not.but was not the mo...
Keywords:Sophora tonkinensis Gagnep    SOD  POD  CAT  germination rate  
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