| 猪肺炎支原体PCR鉴定方法的建立 |
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| 引用本文： | 刘茂军,李冬梅,邵国青,冯志新,吴叙苏,甘源,刘冬霞. 猪肺炎支原体PCR鉴定方法的建立[J]. 金陵科技学院学报, 2008, 24(3) |
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| 作者姓名： | 刘茂军 李冬梅 邵国青 冯志新 吴叙苏 甘源 刘冬霞 |
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| 作者单位： | 江苏省农业科学院兽医研究所,江苏,南京,210014;秦皇岛正大有限公司,河北,山海关,066200 |
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| 基金项目： | 江苏省自然科学基金，江苏省农业三项工程项目，江苏省农业科学院资助项目 |
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| 摘 要： | 根据猪肺炎支原体的P36基因序列设计一对引物,建立猪肺炎支原体PCR检测方法,并对该方法进行了特异性和敏感性试验。成功建立了猪肺炎支原体特异且敏感的PCR检测方法,可区别猪鼻支原体、絮状支原体和鸡毒支原体,最低检出量为4.26 ng。
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| 关 键 词： | 猪肺炎支原体 PCR 鉴定 |
Establishment of PCR Method for the Identification of Mycoplasma hyopneumoniae |
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| LIU Mao-jun,LI Dong-mei,SHAO Guo-qing,FENG Zhi-xin,WU Xu-su,GAN Yuan,LIU Dong-xia. Establishment of PCR Method for the Identification of Mycoplasma hyopneumoniae[J]. Journal of Jinling Institute of Technology, 2008, 24(3) |
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| Authors: | LIU Mao-jun LI Dong-mei SHAO Guo-qing FENG Zhi-xin WU Xu-su GAN Yuan LIU Dong-xia |
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| Abstract: | Aim To establish a PCR method for identification of Mycoplasma hyopneumoniae(Mhp).Method According to the P36 gene of Mhp, two primers were designed.And the specificity and sensibility assay were performed.Result Mhp gene could be Specifically amplified and be different from the genes of Mycoplasma hyorhinitis,Mycoplasma hyorhinitis and Mycoplasma gallisepticum.In addition,the method could detect at least 4.26ng of Mhp genome.Conclusion The PCR method for detecting Mhp was developed with high specificity and sensibility. |
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| Keywords: | Mycoplasma hyopneumoniae PCR identification |
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