首页 | 本学科首页   官方微博 | 高级检索  
     

精子介导法制备转基因鸡的研究
引用本文:苗向阳,丁淑燕,郝慧芳,杨文胜. 精子介导法制备转基因鸡的研究[J]. 中国畜牧兽医, 2007, 34(7): 67-69
作者姓名:苗向阳  丁淑燕  郝慧芳  杨文胜
作者单位:中国农业科学院北京畜牧兽医研究所,北京 100094
基金项目:中国农业科学院院长基金;中国农业科学院资助项目
摘    要:以LacZ基因为报告基因,构建载体质粒p-OV-β-Gal,转移到鸡早期胚胎内,对外源基因的整合及表达情况进行检测分析,为制备转基因鸡探索新的方法。采用脂质体介导法、精子介导法以及电激法相结合的方法将载体质粒p-OV-β-Gal转移到鸡早期胚胎内,进行转基因鸡研究,结果发现其外源基因整合阳性率为13.2%(7/53),表达阳性率为20.7%(11/53)。说明该方法可作为制备转基因鸡的有效方法。

关 键 词:  转基因  精子介导法  
文章编号:1671-7236(2007)07-0067-02
修稿时间:2006-12-21

Production of Transgenic Chicken by Sperm-mediated Method
MIAO Xiang-yang,DING Shu-yan,HAO Hui-fang,YANG Wen-sheng. Production of Transgenic Chicken by Sperm-mediated Method[J]. China Animal Husbandry & Veterinary Medicine, 2007, 34(7): 67-69
Authors:MIAO Xiang-yang  DING Shu-yan  HAO Hui-fang  YANG Wen-sheng
Affiliation:1.Institute of Animal Sciences, Chinese Academy of Agriculture Sciences, Beijing 100094, China; 2.College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China
Abstract:To explore the possibility of generation of transgenic chicken, vector p-OV-β-Gal of LacZ gene as report gene were transmitted into the chicken''s early embryos by sperm-mediated method, then the LacZ gene’s integration and expression were monitored. The vector (p-OV-β-Gal) could be transmitted into the chicken’s early embryos by sperm-mediated method, the percentage of LacZ gene''s integration and expression were respectively 13.2%(7/53)and 20.7%(11/53). The result showed that sperm-mediated method could be used for generation of the transgenic chickens.
Keywords:chicken  transgene  sperm-mediated method
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《中国畜牧兽医》浏览原始摘要信息
点击此处可从《中国畜牧兽医》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号