| 杠柳杀虫活性成分的分离 |
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| 引用本文： | 师宝君,高履桐,姬志勤,张继文,吴文君,胡兆农.杠柳杀虫活性成分的分离[J].农药学学报,2012,14(1):103-106. |
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| 作者姓名： | 师宝君 高履桐 姬志勤 张继文 吴文君 胡兆农 |
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| 作者单位： | 1.西北农林科技大学植物保护学院, 陕西 杨凌 7121002 |
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| 基金项目： | 国家重点基础研究发展计划(973)项目 (2010CB126105); 国家公益性行业(农业)科研专项(200903052); 国家自然科学基金 (30870344,31071704);西北农林科技大学青年学术骨干项目. |
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| 摘 要： | 采用柱层析分离、高效液相色谱切分和生物活性追踪法,从杠柳 Periploca sepium 根皮甲醇提取物中分离出2个具有杀虫活性的化合物(G1和G2),经鉴定其分别为已知物杠柳新苷D和F。生物活性测定结果表明,化合物G1和G2 对3龄粘虫 Mythimna separata 48 h的胃毒致死中浓度(LC50)分别为0.39和0.34 mg/mL, 对小菜蛾 Plutella xyllostella 48 h的胃毒LC50值分别为1.21和1.39 mg/mL。
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| 关 键 词： | 杠柳 杀虫活性 分离 杠柳新苷 |
| 收稿时间： | 2011/7/26 0:00:00 |
| 修稿时间： | 2011/10/23 0:00:00 |
Isolation of the insecticidal ingredients from Periploca sepium |
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| SHI Baojuna,GAO Lütonga,JI Zhiqina,ZHANG Jiwenb, WU Wenjuna,HU Zhaonong.Isolation of the insecticidal ingredients from Periploca sepium[J].Chinese Journal of Pesticide Science,2012,14(1):103-106. |
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| Authors: | SHI Baojuna GAO Lütonga JI Zhiqina ZHANG Jiwenb WU Wenjuna HU Zhaonong |
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| Institution: | 1.College of Plant Protection, Yangling 712100, Shaanxi Province, China2.College of Sciences, Northwest Agricultural and Forestry University, Yangling 712100, Shaanxi Province, China |
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| Abstract: | Two compounds,periplocoside D and F were isolated from the methanol extract of the root barks of Periploca sepium by silica gel chromatograph,high pressure liquid chromatograph(HPLC) and bioassay-guided fractionation.The bioassay results showed that periplocoside D and F had stomach toxic activity with the LC50 value of 48 h 0.39 and 0.34 mg/mL against 3rd instar larvae of Mythimna separata,and against 3rd instar larvae of Plutella xyllostella with the LC50 value of 48 h 1.21 and 1.39 mg/mL. |
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| Keywords: | Periploca sepium insecticidal activity isolated periplocoside |
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