杜仲叶次生代谢物生态学研究初报

通过对不同产地杜仲叶中次生代谢物（ 有效成分）含量的测定及其生态因素分析，对杜仲叶次生代谢物进行了生态学研究，结果表明：不同产地杜仲叶次生代谢物含量差异显著：遵义地区的京尼平甙、绿原酸、桃叶栅瑚甙、总黄酮含量最高，慈利地区的京尼平甙酸含量最高；洛阳地区的京尼平甙含量最低，宜昌地区的绿原酸、桃叶珊瑚甙、总黄酮、京尼平甙酸含量最低，但杜仲胶含量最高，从而说明了生态因素也是调控次生代谢物的重要因素。对各生态因子进行逐步回归分析和通径分析的结果表明：影响杜仲叶次生代谢物合成与积累的主要因素是微量元素；土壤中微量元素（ 有效态） 与杜仲（ 叶） 中微量元素（ 植物吸收） 不完全呈正相关关系；以两者为主因素各进行的逐步回归分析和通径分析的结果不一致。通过系统聚类分析和模糊综合评判得出：贵州遵义、湖南慈利、陕西略阳、四川通江４ 个地区杜仲叶中次生代谢物含量较高

AN ECOLOGICAL STUDY ON SECONDARY METABOLITES OF THE LEAVES OF EUCOMMIA ULMOIDES

An ecological study was carried out on secondary metabolites(bioactive constituents) in the leaves of Eucommia ulmoides through determining the contents of the metabolites and ecological factors in the leaves collected from different regions.The results were as follows:;The contents of metabolites in the leaves collected from different regions showed significant differences.The leaves collected from Zunyi contained the highest contents of geniposide,chlorogenic acid,aucubin,and flavonoids.Geniposidic acid reached the highest content in the leaves from Cili.The leaves from Luoyang showed the lowest content of geniposide.The contents of chlorogenic acid,aucubin,geniposidic acid and flavonoids were the lowest in the leaves from Yichang,but hightest gutta percha.These showed that ecological factor was also an improtant one in regulating secondary metabolism.;Stepwise regression analysis and path analysis indicated that the major factor influencing the synthesis and accumulation of metabolites was the contents of trace elements,meteorological factor however,showed little influence.The relationship between the contents of available trace elements in the soil and in the leaves was not in completely direct correlation.Different results were obtained in stepwise regression analysis and path analyses when the contents of trace elements in the soil and in the leaves were used as the main factors respectively.;Through systematic cluster and fuzzy complex discrimination,it was concluded that the contents of secondary metabolites were high in the leaves from Zunyi(Guizhou Province),Cili(Hunan Province),Lueyang(Shaanxi Province),Tongjiang(Sichuan Province).