| 不同浓度的外源激素对香石竹组织培养的影响 |
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| 引用本文： | 刘开辉,丁小维,邓百万,陈文强,刘飞虎. 不同浓度的外源激素对香石竹组织培养的影响[J]. 安徽农业科学, 2007, 35(19): 5735-5736 |
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| 作者姓名： | 刘开辉 丁小维 邓百万 陈文强 刘飞虎 |
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| 作者单位： | 陕西理工学院陕西省资源生物重点实验室,陕西汉中,723001;云南大学生命科学学院,云南昆明,650091 |
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| 基金项目： | 陕西省教育厅重点科研项目(03JK027) |
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| 摘 要： | 研究了不同浓度的外源激素对香石竹组织培养过程中愈伤组织诱导、芽分化和芽增殖的影响。结果表明,愈伤组织的诱导与BA与NAA浓度比及KT浓度有关;芽诱导与BA、KT、NAA均有关;芽增殖与BA的浓度及BA和NAA的浓度比有关。MS+BA 0.3 mg/L+KT0.5 mg/L+NAA 1.0 mg/L培养基对愈伤组织诱导效果最好;最佳的芽分化培养基为MS+BA 0.5 mg/L+KT 0.5 mg/L+NAA 2.0 mg/L,芽分化率达65%;芽增殖培养基以MS+BA 0.3 mg/L+NAA 0.1 mg/L为最佳,增殖芽数最多,玻璃化率最低。
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| 关 键 词： | 香石竹 组织培养 激素 |
| 文章编号： | 0517-6611（2007）19-05735-02 |
| 修稿时间： | 2007-03-05 |
Effect of Exogenous Hormone with Different Concentration on Tissue Culture of Dianthus caryophyllus |
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| LIU Kai-hui et al;. Effect of Exogenous Hormone with Different Concentration on Tissue Culture of Dianthus caryophyllus[J]. Journal of Anhui Agricultural Sciences, 2007, 35(19): 5735-5736 |
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| Authors: | LIU Kai-hui et al |
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| Affiliation: | Shaanxi University of Technology; Shaanxi Key Laboratory of Resource Biology; Hanzhong; Shaanxi 723001 |
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| Abstract: | The analysis on the effects of exogenous hormone with different concentration on callus initiation,bud differentiation and bud multiplication showed that callus initiation had correlation to the concentration ratio of BA and NAA, and KT concentration.The bud initiation related to BA,KT and NAA.The bud multiplication related to the concentration of BA and concentration ratio of BA and NAA.MS medium supplemented with 0.3 mg/L BA,0.5 mg/L KT and 1.0 mg/L NAA had the best effect on callus initiation.The preferable medium for bud differentiation was MS medium supplemented with 0.5 mg/L BA,0.5 mg/L KT and 2.0 mg/L NAA,and the preferable medium for bud multiplication was MS medium supplemented with 0.3 mg/L BA and 0.1 mg/L NAA.It had most multiplication buds and the lowest vitrification rate. |
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| Keywords: | Dianthus caryophyllus Tissue culture Hormone |
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