| 多羟基双萘醛(WCT)抗血清的制备与不同酶联免疫吸附检测法的对比试验 |
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| 引用本文： | 黎登寨,吴云锋,杨旭光,王睿,崔晓艳.多羟基双萘醛(WCT)抗血清的制备与不同酶联免疫吸附检测法的对比试验[J].西北农业学报,2004,13(3):48-51. |
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| 作者姓名： | 黎登寨 吴云锋 杨旭光 王睿 崔晓艳 |
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| 作者单位： | 西北农林科技大学,陕西杨凌,712100 |
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| 基金项目： | 陕西省自然科学基金(2002SM16) |
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| 摘 要： | 通过对多羟基双萘醛分子式上的醛基的改造,利用混合酸酐法使小分子化合物多羟基双萘醛能够与大分子蛋白质BSA相结合,形成人工抗原,注射兔子,成功制备WCT抗血清。通过ELISA检测,血清的效价为1∶256,非专化性效价为1∶2。本试验比较了酶联夹心法和间接酶联法的灵敏度,发现间接法检测WCT的灵敏度比夹心法相对高,这为应用间接法检测WCT提供了依据。
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| 关 键 词： | 多羟基双萘醛 抗血清 ELISA |
| 文章编号： | 1004-1389(2004)03-0048-04 |
| 收稿时间： | 2004/4/1 0:00:00 |
| 修稿时间： | 2004年4月1日 |
Antiserum Preparation and Differ-ELISA Detection for Polyhydroxy Dinaphthaldehyde(WCT) |
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| LI Deng-zhai,WU Yun-feng,YANG Xu-guang,WANG Rui and CUI Xiao-yan.Antiserum Preparation and Differ-ELISA Detection for Polyhydroxy Dinaphthaldehyde(WCT)[J].Acta Agriculturae Boreali-occidentalis Sinica,2004,13(3):48-51. |
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| Authors: | LI Deng-zhai WU Yun-feng YANG Xu-guang WANG Rui and CUI Xiao-yan |
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| Institution: | Northwest Sci-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China;Northwest Sci-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China;Northwest Sci-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China;Northwest Sci-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China;Northwest Sci-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China |
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| Abstract: | The manual antigen was prepared by modified the aldehyde of WCT and linking the small molecular compound-WCT and the larger molecular protein-BSA by the way of mixing acid anhydride.Then injected into the rabbit to prepare the antiserum of WCT.The titer of antiserum and nonspecific reached to 1:256 and 1:2 respectively by ELISA detection.Compared the sensitivity of enzyme-linked sandwich assay and indirect enzyme-linked assay,the latter is more applicable.This provided the evidence to detect WCT using the indirect enzyme-linked assay. |
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| Keywords: | WCT Antiserum ELISA |
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