Application of real-time PCR for early diagnosis of diseases caused by Aeromonas salmonicida,Vibrio anguillarum,and Tenacibaculum maritimum in turbot: A field study |
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| Authors: | María-José Chapela Martiña Ferreira Asela Ruiz-Cruz Iris Martin-Varela Jacobo Fernández-Casal Alejandro Garrido-Maestu |
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| Affiliation: | 1. Department of Health-Nutrition and Pharma, ANFACO-CECOPESCA, Vigo (Pontevedra), Spainalejandro.garrido@inl.int;3. Department of Resources and Aquaculture, ANFACO-CECOPESCA, Vigo (Pontevedra), Spain;4. INSUI?A S.L., Xove, Spain;5. Department of Life Sciences, Nano4Food Unit, Food Quality and Safety Research Group, International Iberian Nanotechnology Laboratory, Braga, Portugal |
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| Abstract: | In the present study a multiplex real-time PCR method was developed for early detection of diseased fish infected by Aeromonas salmonicida, Vibrio anguillarum, and/or Tenacibaculum maritimum. The method consisted of the detection of three species-specific genes after DNA extraction with a commercial kit. Three types of samples were tested, and the results were compared with those of traditional diagnosis. The method obtained a limit of detection of 104 cfu/mL (2 x 102 cfu/tube). Additionally, 27 samples from fish showing signs of disease were correctly diagnosed by the developed methodology, demonstrating its suitability for implementation in aquaculture. |
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| Keywords: | Multiplex qPCR diagnosis bacterial diseases aquaculture |
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