Osmoregulation in birdseed millet under conditions of water stress II. Variation in F3 lines of Setaria italica and its relationship to plant morphology and yield

We report, for the first time, there generation of four homozygous lines in sweet cherry (Prunus avium L.) byin situ parthenogenesis followed by embryo and cotyledon culture. The sweet cherry cultivar ‘Altenburger’ was pollinated with marked pollen irradiated by γ-rays at doses ranging from 250 to1200 Gy. Pollination with such irradiated pollen affected fruit set and the quality of the embryos, and induced the formation of parthenogenic embryos. The immature embryos extracted from the stones, 35 or 75days after pollination, were cultivatedin vitro in an embryo or cotyledon culture. Although flow cytometrical analysis demonstrated the diploid level for all regenerants, four lines could be characterized as homozygous using isoenzyme analysis. This revised version was published online in July 2006 with corrections to the Cover Date.