铁皮石斛高效再生体系研究与应用

以原球茎为实验材料,对影响铁皮石斛再生体系的主要影响因素进行试验,建立铁皮石斛在生产中的高效再生体系。试验结果表明,在基本培养基MS、Fonnesbech和N6中,最适合的基本培养基为MS;原球茎最佳增殖培养基为MS＋6-BA0．75mg／L＋NAA0．2mg／L＋4．0％白糖,接种35d后原球茎增殖倍数为4．19;最佳分化培养基为Ms＋6-BA0．3mg／L＋3．0％白糖,分化率为74％。分化出的丛芽在生根培养基MS＋AC0．05％＋白糖3．0％中,28d生根率达到87％。

Development and Application of Efficient Regeneration System for Dendrobium officinale

Protocorms of Dendrobium officinale were used as materials to determine main factors affecting the regeneration system for D. officinale, based on which a highly efficient regeneration system for D. officinale was developed. The results showed that the best basic culture medium was MS among MS, Fonnesbech （1972） and N6 （1974） tried; that the best multiplication culture medium was MS＋6-BA0.75 mg/L＋NAA0.2 mg/L＋4.0% white sugar, with multiplication rate of the protocorms being 4.19 times 35 days after inoculation; that the best differentiation culture medium was MS＋6-BA0.3 mg/L＋3.0% white sugar, with 74% of differentiation rate; and that differentiated cluster shoots had a rooting rate of 87% when cultured on the rooting medium （MS＋AC0.05%＋white sugar 3.0%） for 28 days.