甘薯对马铃薯腐烂线虫分泌物的反应

 马铃薯腐烂线虫(Ditylenchus destructor)分泌物滴注到甘薯感病品种薯块内,孔壁周围组织出现褐变和轻度腐烂。同样方法处理抗病品种,仅孔壁变褐。石蜡切片检查发现感病品种褐变组织细胞离析崩解,而抗病品种孔壁上的薄壁细胞形成木栓层,与接种该线虫悬浮液的反应是一致的。将分泌物定量滴加到抗病和感病品种的甘薯片上,结果抗病愈伤组织被刺激产生肿瘤,组织增生,感病品种无这种现象。经组织切片染色镜检,肿瘤部分的细胞比正常增大2~3倍,无淀粉粒和晶簇,但细胞壁不破裂。处理30天,愈伤组织的肿瘤表层细胞发展成木栓层。应用SDS-聚丙烯酰胺凝胶电泳法测定,分泌物含有18种蛋白质,最大分子量为93000,最小分子量为16000。

RESPONSES OF SWEET POTATO TO EXUDATE OF POTATO ROT NEMATODES (DITYLENCHUS DESTRUCTOR)

The tuber roots of susceptible sweet potato were injected with exudate from D. destructor. The tissue aroud inoculated holes about 1~1.5cm became brown and light rot. The hole walles of resistant variety were brown only by same method, The cells of brown tissues of suscebtible variety were booken down and disrupted;parenchyma cells of hole wall of resistant variety were induced to produce cork layer. The responses were the same as those inoculated with the nematode suspension. The section of sweet potato of resistant and susceptible varieties were added quantitatively with exudate of D. destructor. Resistant callus was stimulated to form giant gall and tissue hyperplasia. There was no such phenomenon in susceptible callus and contrast. Parenchyma cells of gall were three and four times larger than normal cells. There were few starch particles and crystals in the cells of gall. But the cell walls were not collapse The cells of surface layer of gall were stimulateb to form cork layer after treated for 30 days. Analysis of the exudate by soldium dodecyl sulfate-polyacy-rylamied gel electrophoresis revealed the presence of eighteen protein bands with silver strain. Based upon the molecular weights of commercial standards, the proteins in exudate ranged from 16k to 93k daltons.