高通量测序分析人工养殖成年林麝粪便古菌菌群多样性

本试验的目的是采用高通量测序技术研究人工养殖成年林麝粪便中古菌的结构和组成,并对比不同性别之间的差异。选取12只3岁健康的林麝依据性别分为雄性组(M组)和雌性组(F组),每组各6只。采集其新鲜粪便,提取DNA,用古菌通用引物PCR扩增古菌16S rRNA的V4~V5区,用M i Seq 300 PE测序平台对扩增产物进行高通量测序,用QIIM E等软件对测序序列进行分析统计。结果表明:在从门到属的各级分类水平上,F组与M组古菌的相对丰度差异均不显著(P0.05)。M组和F组的组内遗传距离分别为0.16±0.03和0.27±0.06,组间为0.24±0.07,样品的相似度很高。林麝粪便中的古菌可以分为3个门,优势门为阔古细菌门(Euryarchaeota);在属水平上可以分为7个已知属,优势属为甲烷短杆菌属(Methanobrevibacter),其次为热裸单胞菌属(Thermogymnomonas)。结果提示,雄性和雌性林麝粪便中古菌的结构和组成都没有显著差异,Methanobrevibacter是林麝粪便中的优势古菌属

Fecal Archaeal Diversity of Artificial Breeding Adult Forest Musk Deer Analyzed by High-Throughput Sequencing Technologies

This experiment was conducted to invest the archaeal structure and composition in feces of artificial breeding adult forest musk deer ( FMD) using high-throughput sequencing technologies, and to compare the difference between male and female FMD.Twelve healthy FMD with the age of three years were divided into male group ( M) and female group ( F) according to their gender ( 6 deer in each group) .The fresh feces were collected and DNA was extracted from them.The archaeal universal primers were used to amplify V4 to V5 regions of archaeal 16S rRNA.The amplifications were high-throughput sequenced with the MiSeq 300PE sequencing platform.The QIIME and other softwares were used to analyze the data.The results showed as fol-lows: from phylum to genus levels, the differences of the archaeal relative abundance between group M and group F were not significant ( P>0.05 ) .Genetic distance within the samples in group F and group M was 0.16±0.03 and 0.27 ±0.06, respectively, and that between groups was 0.24 ±0.07; all samples shared high similarity.The archaea in the feces of FMD can be divided into three phyla, the dominant phylum was Eur-yarchaeota; on the genus level they can be divided into seven existing genera, the dominant genus was Metha-nobrevibacter, the next one was Thermogymnomonas.In conclusion, the gender has no significant effect on the archaeal structure and composition in the feces of FMD, and Methanobrevibacter is the dominant genus.