HPLC法测定益肾乌发口服液中二苯乙烯苷的含量

目的]建立测定益肾乌发口服液中2,3,5,4′-四羟基二苯乙烯-2-Oβ--D葡-萄糖苷含量的方法。方法]采用HPLC色谱法对主药何首乌的主要成分2,3,5,4′-四羟基二苯乙烯-2-Oβ--D葡-萄糖苷的含量进行定量分析,色谱柱为岛津VP-ODsc（250.0 mm×4.6 mm,5μm）,流动相为乙腈-水（25∶75）;流速1.2 m l/m in,检测波长为320 nm。结果]2,3,5,4′-四羟基二苯乙烯-2-Oβ--D葡-萄糖苷在0.487~13.489 mg范围内呈良好的线性关系,加样回收率为99.94%,RSD=1.75%（n=6）。结论]高效液相色谱法测定益肾乌发口服液中2,3,5,4′-四羟基二苯乙烯-2-Oβ--D葡-萄糖苷的含量方法操作简单、准确、重复性好、精密度高、专属性强,可以作为益肾乌发口服液的质量控制标准。

Determination of Stilbene Glucoside in Yishenwufa Oral Solution by HPLC

Objective]The research aimed to establish a method for the determination of stilbene glucoside in Yishenwufa oral solution by HPLC.Method]The content of stilbene glucoside in Yishenwufa oral solution was determined by HPLC.The separation was performed on a VP-ODS c-c18 column(250.0 mm×4.6 mm,5 μm).The mobile phase was cetonitrile-water(25∶75) at the flow rate of 1.2 ml/min.The defection wavelength was 320 nm.Result]The linear range of 2 styrene glucose glucoside was in the range of 0.487-13.489 mg;the addition recovery rate was 99.94%,RSD=1.75%(n=6).Conclusion]The method of the determination of stilbene glucoside in Yishenwufa oral solution is simple,rapid,good repeatability,high precision,strong specificity,and it can be determination standard of the content of Yishenwufa oral solution.